Mouse anti vimentin

Manufactured by Proteintech

Sourced in United States

Mouse anti-Vimentin is a primary antibody that recognizes the intermediate filament protein vimentin. Vimentin is commonly used as a marker for mesenchymal cells and is involved in maintaining cell structure and integrity.

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Vimentin (336 citations) Anti-vimentin (129 citations) Mouse monoclonal anti-Vimentin (3 citations) Anti-TM (2 citations) Mouse-anti-Vimentin antibody (2 citations)

4 protocols using mouse anti vimentin

Antibody Characterization for Cell Signaling

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Rabbit anti-ACAT2 (1:1000) (ab131215) and rabbit anti-P21 (1:1000) (ab109520) were purchased from Abcam. Rabbit anti-SETD7 antibody(1:1000) (24840-1-AP), rabbit anti-P16-INK4A(1:1000) (10883-1-AP), rabbit anti-P27 (1:1000)(25614-1-AP), rabbit anti-Cyclin B1 (1:1000)(55004-1-AP), rabbit anti-Cyclin E1(1:1000)(11554-1-AP), mouse anti-Cyclin D1(1:1000)(60186-1-Ig), rabbit anti-MCM2(1:1000)(10513-1-AP), rabbit anti-Cortactin (1:400)(11381-1-AP) and rabbit anti-SMA (1:1000)(14395-1-AP), mouse anti-Vimentin(1:4000)(60330-1-Ig) were purchased from Proteintech. Rabbit anti-Snail2 (1:1000) (121235) was purchased from Brickell Biotech, Inc. Antibodies against YAP1(1:1000) (A1002), TAZ (1:1000) (A23034), TEAD1(1:1000) (A5218) were purchased from AB clonal. Rabbit anti-vinculin (1:1000) (E1E9V), rabbit anti-flag antibody (1:1000) (2272S), rabbit anti-myc antibody (1:1000) (14793S), rabbit anti-p53 (7F5) (1:1000)(2527S), rabbit anti-E-Cadherin (24E10) (1:1000)(3195S), rabbit anti-N-Cadherin (D4R1H) XP®(1:1000)(13116S) and mouse anti-ubiquitin (1:1000) (#3936) were purchased from Cell Signalling Technology.
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).

Zhang M., Cai F., Guo J., Liu S., Ma G., Cai M., Zhang R, & Deng J. (2024). ACAT2 suppresses the ubiquitination of YAP1 to enhance the proliferation and metastasis ability of gastric cancer via the upregulation of SETD7. Cell Death & Disease, 15(4), 297.

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Immunofluorescence Analysis of IPF Lung

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Paraffin sections or cryosections of lung tissues from IPF patients and pulmonary fibrosis mice were utilized for immunofluorescence staining. The following primary antibodies were employed: Rabbit anti-Bcar3 (1:100, Proteintech, Wuhan, China), mouse anti-PDGFR-β (1:100, Santa Cruz, CA, USA), mouse anti-a-SMA (1:100, Santa Cruz, CA, USA), and mouse anti-Vimentin (1:100, Proteintech, Wuhan, China). To visualize the labeled proteins, Alexa 594- or 488-conjugated anti-rabbit or anti-mouse antibodies (1:400, Abbkine, CA, USA) were utilized as fluorescence secondary antibodies, and DAPI was employed to counterstain the nuclei. Fluorescence images were captured using a fluorescence microscope (Olympus, Shinjuku, Japan).

Zeng C., Wang Q., Liu X., Wang K., Wang C., Ju X., Wang T., Zhou Q., Fu X., Yu J, & Wang Y. (2024). Localized Administration of Bcar3 siRNA via Nano-Self-Assembly to Treat Idiopathic Pulmonary Fibrosis by Disrupting Macrophage-Fibroblast Crosstalk. International Journal of Nanomedicine, 19, 1827-1842.

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Antibody Validation Protocol for Protein Expression

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In this study, we used the following antibodies: Rabbit anti-MEIS1(Atlas) ,Rabbit anti-MEIS2 (Proteintech), Rabbit anti-MEIS3(Atlas), Rabbit anti-LAMB1(Proteintech), Rabbit anti-MMP2 (Proteintech), Rabbit anti-E-Cadherin(CST), Mouse anti-Beta Catenin(Proteintech), Mouse anti-Vimentin (Proteintech), Rabbit anti-ACTB(Proteintech), HRP-conjugated A nipure Donkey Anti-Mouse IgG(H+L) (Proteintech), HRPconjugated Donkey Anti-Rabbit IgG(H+L)(Proteintech), Rabbit anti-E-Cadherin (Thermo Fisher Scienti c) and Mouse anti-Vimentin(CST). Cell culture medium and serum were purchased form Thermo Fisher Scienti c.

Wang W., Li H., Wang M., Zhu M., Li A., & Ling Y. (2021). High MEIS3 expression intimates poor prognosis for clinical stage II/III colorectal cancer patients.

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Antibody Validation Protocol for Protein Expression

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Li H., Ma J., & Ling Y. (2022). High MEIS3 level in colorectal cancer of stage II/III indicates poor prognosis.

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