Cell viability kit

Manufactured by Abcam

The Cell Viability Kit is a laboratory assay that measures the number of viable cells in a sample. The kit uses a colorimetric method to quantify the metabolic activity of cells, which serves as an indicator of cell viability and proliferation.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (4 mentions) Infinity reagent, by Thermo Fisher Scientific (1 mentions) Multiplex bead array, by Eve Technologies (1 mentions)

Close spelling variants of this product

EZViable Calcein AM Fluorometric Cell Viability Assay Kit Cell viability assay kit ApoSENSOR™ ATP Cell Viability Bioluminescence Assay Kit Neutral Red Assay Kit—Cell Viability/Cytotoxicity ApoSENSOR cell viability assay kit VisionBlue Quick Cell Viability Fluorometric Assay Kit Live/dead cell viability assay kit Resazurin Cell Viability Assay kit MTS colorimetric cell viability kit

2 protocols using cell viability kit

Liver ATP and ALT Assays

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ATP assay. Absolute levels of adenosine triphosphate (ATP) were determined in liver biopsies with a luminescence-based assay (Cell Viability Kit; Biovision). Briefly, nucleotides were extracted from ~1 mg of pulverized tissue and peak luminescence was measured for 60 seconds in a single tube. ATP concentrations were normalized to tissue protein content determined by the BCA protein assay (Thermo Fisher Scientific, Waltham, MA).
Alanine transaminase assay. ALT concentration was determined in the perfusate by assessing the enzymatic conversion of L-alanine by ALT and LDH in a kinetic assay (Infinity Reagent; Thermo Fisher Scientific).

Bruinsma B.G., Sridharan G.V., Weeder P.D., Avruch J.H., Saeidi N., Özer S., Geerts S., Porte R.J., Heger M., van Gulik T.M., Martins P.N., Markmann J.F., Yeh H, & Uygun K. (2016). Metabolic profiling during ex vivo machine perfusion of the human liver. Scientific Reports, 6, 22415.

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Biomarker Assessment in Liver Samples

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Alanine transaminase (ALT) release was determined in the blood using a point-of-care blood chemistry analyzer (Piccolo, Abaxis, Union City, CA). Levels of C-reactive protein (CRP), TNF- α, IL-1β, IFN-γ and IL-12 were determined in the blood using a multiplex bead array (Eve Technologies, Calgary, Canada). Absolute concentration of adenosine triphosphate (ATP) was measured in liver samples using a luminescence-based assay (Cell Viability Kit; Biovision). Nucleotides were extracted from ~1 mg of homogenized tissue, after which peak luminescence was determined. ATP concentrations were normalized to tissue protein using a BCA protein assay (Thermo Fisher Scientific, Waltham, MA).

Avruch J.H., Bruinsma B.G., Weeder P.D., Sridharan G.V., Porte R.J., Yeh H., Markmann J.F, & Uygun K. (2017). A novel model for ex situ reperfusion of the human liver following subnormothermic machine perfusion. Technology, 5(4), 196-200.

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