Log In Sign up
The largest database of trusted experimental protocols

229e spike

Manufactured by Sino Biological
Visit Supplier

The 229E spike is a laboratory product that represents the spike protein of the 229E strain of the human coronavirus. It is a recombinant protein produced in a mammalian cell expression system. The 229E spike protein is a key structural component of the coronavirus and is involved in the virus's attachment and entry into host cells.

Automatically generated - may contain errors

Lab products found in correlation

Mers spike, by Sino Biological (3 mentions) Nl63 spike, by Sino Biological (3 mentions) Hku1 spike, by Sino Biological (3 mentions) Oc43 spike, by Sino Biological (3 mentions) Goat anti human igg alexa fluor 647, by Jackson ImmunoResearch (3 mentions) Ique screener plus, by Intellicyt (2 mentions) Anti human iga alexa fluor 647, by Jackson ImmunoResearch (2 mentions) Anti human igm alexa fluor 647, by Jackson ImmunoResearch (2 mentions)

Close spelling variants of this product

Recombinant human coronavirus 229E spike protein (2 citations)

3 protocols using 229e spike

1

Multiplex Serological Profiling of SARS-CoV Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptavidin-coated beads with different intensities of phycoerythrin (PE)-channel fluorescence (Spherotech SVFA-2558–6K and SVFB-2558–6K) were incubated with the following biotinylated antigens: 10 μg/mL MERS spike (Sino Biological 40069-V08B), NL63 spike (Sino Biological 40604-V08B), 229E spike (Sino Biological 40605-V08B), HKU1 spike (Sino Biological 40606-V08B), OC43 spike (Sino Biological 40607-V08B), SARS-CoV-2 spike, SARS-CoV-2 RBD, SARS-CoV-2 NTD, SARS-CoV-1 spike, SARS-CoV-1 RBD or 10 μg/mL CD4 (56 (link)) as a control. SARS-CoV-2 and SARS-CoV-1 antigens were produced in-house as described above. Excess streptavidin sites were blocked with 10 μg/mL of CD4 and the beads were washed and mixed. The beads were stained with 1:50, 1:250 or 1:1250 plasma for 30 minutes at room temperature, washed, and stained with 2.5 μg/mL goat anti-human IgG Alexa Fluor 647 (Jackson Immunoresearch 109-606-170), anti-human IgA Alexa Fluor 647 (Jackson Immunoresearch 109-606-011) or anti-human IgM Alexa Fluor 647 (Jackson Immunoresearch 109-606-129). The samples were read with the iQue Screener Plus (Intellicyt) high-throughput flow cytometer and FACS data were analysed with Flowjo. Data from titrations were analysed by calculating area under the curve (AUC) for the titration and subtracting the AUC of the negative control antigen.
Cho H., Gonzales-Wartz K.K., Huang D., Yuan M., Peterson M., Liang J., Beutler N., Torres J.L., Cong Y., Postnikova E., Bangaru S., Talana C.A., Shi W., Yang E.S., Zhang Y., Leung K., Wang L., Peng L., Skinner J., Li S., Wu N.C., Liu H., Dacon C., Moyer T., Cohen M., Zhao M., Lee F.E., Weinberg R.S., Douagi I., Gross R., Schmaljohn C., Pegu A., Mascola J.R., Holbrook M., Nemazee D., Rogers T.F., Ward A.B., Wilson I.A., Crompton P.D, & Tan J. (2021). Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern. Science Translational Medicine, 13(616), eabj5413.
+ Open protocol
+ Expand
2

SARS-CoV-2 Serology Assay Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptavidin-coated beads with different intensities of PE-channel fluorescence (Spherotech SVFA-2558–6K and SVFB-2558–6K) were incubated with the following biotinylated antigens: 10 μg/mL MERS spike, NL63 spike, 229E spike, HKU1 spike, OC43 spike (all from Sino Biological), SARS-CoV-2 spike, SARS-CoV-2 RBD, SARS-CoV-2 NTD, SARS-CoV-1 spike, SARS-CoV-1 RBD or 10 μg/mL CD4 as a control. Excess streptavidin sites were blocked with 10 μg/mL of CD4 and the beads were washed and mixed. The beads were stained with 1/50, 1/250 or 1/1250 plasma for 30 min at room temperature, washed, and stained with 2.5 μg/mL goat anti-human IgG Alexa Fluor 647 (Jackson Immunoresearch 109-606-170). The samples were read with the iQue Screener Plus (Intellicyt) high-throughput flow cytometer and FACS data were analysed with Flowjo. Data from titrations were analysed by calculating area under the curve (AUC) for the titration and subtracting the AUC of the negative control antigen.
Cho H., Gonzales-Wartz K.K., Huang D., Yuan M., Peterson M., Liang J., Beutler N., Torres J.L., Cong Y., Postnikova E., Bangaru S., Talana C.A., Shi W., Yang E.S., Zhang Y., Leung K., Wang L., Peng L., Skinner J., Li S., Wu N.C., Liu H., Dacon C., Moyer T., Cohen M., Zhao M., Lee F.E., Weinberg R.S., Douagi I., Gross R., Schmaljohn C., Pegu A., Mascola J.R., Holbrook M., Nemazee D., Rogers T.F., Ward A.B., Wilson I.A., Crompton P.D, & Tan J. (2021). Ultrapotent bispecific antibodies neutralize emerging SARS-CoV-2 variants. bioRxiv.
+ Open protocol
+ Expand
3

Multiplexed SARS-CoV Antigen Binding Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptavidin-coated beads with different intensities of phycoerythrin (PE)–channel fluorescence (Spherotech, SVFA-2558-6K and SVFB-2558-6K) were incubated with the following biotinylated antigens: 10 μg/ml of Middle East respiratory syndrome (MERS) spike (Sino Biological, 40069-V08B), NL63 spike (Sino Biological, 40604-V08B), 229E spike (Sino Biological, 40605-V08B), HKU1 spike (Sino Biological, 40606-V08B), OC43 spike (Sino Biological, 40607-V08B), SARS-CoV-2 spike, SARS-CoV-2 RBD, SARS-CoV-2 NTD, SARS-CoV-1 spike, and SARS-CoV-1 RBD or CD4 (10 μg/ml) (56 (link)) as a control. SARS-CoV-2 and SARS-CoV-1 antigens were produced in-house as described above. Excess streptavidin sites were blocked with CD4 (10 μg/ml), and the beads were washed and mixed. The beads were stained with 1:50, 1:250, or 1:1250 plasma for 30 min at room temperature, washed, and stained with 2.5 μg/ml of goat anti-human IgG Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-170), anti-human IgA Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-011), or anti-human IgM Alexa Fluor 647 (Jackson ImmunoResearch, 109-606-129). The samples were read with the iQue Screener Plus (IntelliCyt) high-throughput flow cytometer, and FACS data were analyzed with FlowJo. Data from titrations were analyzed by calculating the area under the curve (AUC) for the titration and subtracting the AUC of the negative control antigen.
Cho H., Gonzales-Wartz K.K., Huang D., Yuan M., Peterson M., Liang J., Beutler N., Torres J.L., Cong Y., Postnikova E., Bangaru S., Talana C.A., Shi W., Yang E.S., Zhang Y., Leung K., Wang L., Peng L., Skinner J., Li S., Wu N.C., Liu H., Dacon C., Moyer T., Cohen M., Zhao M., Lee F.E., Weinberg R.S., Douagi I., Gross R., Schmaljohn C., Pegu A., Mascola J.R., Holbrook M., Nemazee D., Rogers T.F., Ward A.B., Wilson I.A., Crompton P.D, & Tan J. (2021). Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern. Science Translational Medicine, 13(616), eabj5413.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!