We purchased ω-Agatoxin TK, AP-5, CGP 55845A and DNQX from Tocris (Bristol, UK); 1,2-Bis(2-Aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA), Nifedipine and TTX from Sigma (St. Louis, MO); ω-Conotoxin GVIA from AnaSpec (Fremont, CA); and ethanol from Remet (La Mirada, CA). CRF was synthesized by Dr. Jean Rivier at the Salk Institute for Biological Studies, and R121919 was synthesized by Dr. Kenner Rice at the Drug Design and Synthesis Section of the National Institute on Drug Abuse. Drugs were dissolved in aCSF and applied locally by Y-tubing (Murase et al., 1989 (link)) or bath perfusion.
ω agatoxin tk
Manufactured by Bio-Techne
ω-Agatoxin TK is a peptide isolated from the venom of the funnel-web spider Agelenopsis aperta. It functions as a potent and selective blocker of P-type voltage-gated calcium channels.
Automatically generated - may contain errors
Lab products found in correlation
Nifedipine, by Merck Group (2 mentions)
Fluo 4 am, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Cgp 55845a, by Bio-Techne (1 mentions)
1.2 bis 2 aminophenoxy ethane n n n n tetraacetic acid bapta, by Merck Group (1 mentions)
Bilirubin, by Merck Group (1 mentions)
Snx 482, by Bio-Techne (1 mentions)
Hemin, by Merck Group (1 mentions)
Ethylene glycol bis 2 aminoethylether n n n n tetraacetic acid, by Merck Group (1 mentions)
Cell culture media and supplements, by Thermo Fisher Scientific (1 mentions)
ω conotoxin gvia, by Bio-Techne (1 mentions)
Antimycin a, by Merck Group (1 mentions)
Corm 2, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Mg132, by Enzo Life Sciences (1 mentions)
2 protocols using ω agatoxin tk
1
Pharmacological Manipulation of Neurotransmission
2
Investigating CO-mediated Cellular Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell culture media and supplements were purchased from Invitrogen Life Technologies (Carlsbad, USA). Fetal bovine serum (FBS) was obtained from HyClone Laboratories (Logan, UT). MG132 and proteinase inhibitor cocktail were purchased from Enzo Life Sciences (Ann Arbor, USA). CORM-2 (a CO-releasing compound, [Ru(CO)3Cl2]2), RuCl3, RuCl2(DMSO)4, nifedipine, ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetra-acetic acid (EGTA), antimycin A, cycloheximide, hemin, and bilirubin were purchased from Sigma (St. Louis, USA). Sn(IV) protoporphyrin IX dichloride (SnPP) was purchased from Frontier Scientific (Logan, UT). ω-Conotoxin GVIA, ω-Agatoxin TK, and SNX 482 were purchased from Tocris bioscience (Bristol, BS110QL). The 4′,6-Diamidino-2-phenylindole (DAPI) was obtained from Thermo Fisher Scientific (Waltham, USA). Fluo-4 AM was purchased from Thermo Fisher Scientific (Waltham, USA). Compound C (dorsomorphin), an AMPK inhibitor, was purchased from Enzo Life Sciences (Farmingdale, NY, USA).
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