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Trimethylsulfonium hydroxide tmsh

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Trimethylsulfonium hydroxide (TMSH) is a reagent commonly used in analytical chemistry laboratories. It is a quaternary ammonium compound that functions as a methylating agent, enabling the derivatization of various organic compounds for improved detectability and analysis.

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2 protocols using trimethylsulfonium hydroxide tmsh

1

Comprehensive Phytochemical Analysis Protocol

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δ-tocopherol, (+)-γ-tocopherol, (±)-α-tocopherol, D-α-tocotrienol, D-δ-tocotrienol, γ-tocotrienol (analytical standards), methyl palmitate (≥99.0%), methyl stearate (≥99.5%), methyl oleate (≥99.0%), methyl linoleate (≥98.5%), methyl linolenate (≥99.0%), betulinic (≥98%), oleanolic (≥97%), ursolic (≥90%) acids, α-amyrin (≥98%), β-amyrin (≥98.5%), lupeol (≥94%), chlorogenic (≥95%), neochlorogenic (≥98%) acids, kaempferol (≥90%), apigenin (≥97%), myricetin (≥96%), quercetin (≥95%), 2-propanol (99.9%), hexane (≥95%), gradient grade acetonitrile, methanol. and ethanol, trifluoroacetic acid (≥99%), and trimethylsulfonium hydroxide (TMSH) (0.25 M methanolic solution) were purchased from Sigma Aldrich (St. Louis, MO, USA). lupeol acetate (≥98%), α-amyrin acetate (≥98%), and β-amyrin acetate (≥98%) were provided by ALB Technology (ALB Materials Inc, Henderson, NV, USA). Water for HPLC was purified by ULTRAPURE Millipore Direct-Q® 3UV-R (Merck Millipore, Billerica, MA, USA). Tert-butyl methyl ether (TBME) (99.8%) was purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland).
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2

Fatty Acid Profiling of Bacteria

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Membrane fatty acid (FA) composition of C. maltaromaticum was determined on the cell pellets of thawed, washed bacterial samples, according to the method detailed by Meneghel et al. [2] (link). Briefly, lipid extraction was performed using an accelerated solvent extractor (ASE 350, Dionex; Sunnyvale, CA, USA), using a chloroform/methanol-based extraction method. The obtained lipids were then mixed with a C9:0 internal standard (Sigma-Aldrich; St. Louis, MO, USA) and methylated using trimethylsulfonium hydroxide (TMSH, Sigma-Aldrich; St. Louis, MO, USA). FA identification and quantification was carried out on a Hewlett-Packard 6890 gas chromatograph (GMI; Ramsey, MI, USA) coupled to a mass spectrometer (5973, Agilent Technologies; Avondale, PA, USA). Results were expressed as relative FA percentages.
Fatty acid compositions of the L. bulgaricus strains were recalculated from raw data available from Meneghel et al. [2] (link).
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