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Hexadecyl paf

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Hexadecyl PAF is a laboratory reagent used for research purposes. It is a synthetic compound that mimics the structure and function of platelet-activating factor (PAF), a naturally occurring lipid mediator involved in various biological processes. Hexadecyl PAF can be used in experiments to study the role of PAF in cellular signaling, inflammation, and other related research areas.

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Lab products found in correlation

Unlabeled paf 1 o hexadecyl 2 o acetyl sn glycero 3 phophorylcholine, by Enzo Life Sciences (2 mentions) 1 o hexadecyl 2 o acetyl 3h n, by PerkinElmer (2 mentions) Bio safe 2, by Research Products International (2 mentions)

2 protocols using hexadecyl paf

1

Radioisotope-labeled PAF Hydrolase Assay

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The PAF hydrolase activity assay was performed as previously described14 (link) with modifications using [3H]PAF (Platelet Activating Factor, 1-O-Hexadecyl-[Acetyl-3H(N)]-, Hexadecyl PAF) as a substrate. Unlabeled PAF (1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phophorylcholine) was purchased from Enzo Life Sciences, 1-O-Hexadecyl-2-O-[Acetyl-3H(N)]-, Hexadecyl PAF, [acetyl-3H]-, (250μCi (9.25MBq) was purchased from Perkin Elmer, and Bio-Safe II was purchased from Research Products International Corp, Mount Prospect, IL.
He S., Chousterman B.G., Fenn A., Anzai A., Nairz M., Brandt M., Hilgendorf I., Sun Y., Ye Y.X., Iwamoto Y., Tricot B., Weissleder R., Macphee C., Libby P., Nahrendorf M, & Swirski F.K. (2015). Lp-PLA2 Antagonizes Left Ventricular Healing After Myocardial Infarction by Impairing the Appearance of Reparative Macrophages. Circulation. Heart Failure, 8(5), 980-987.
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2

PAF Hydrolase Activity Assay with [3H]PAF

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The PAF hydrolase activity assay was performed as previously described14 (link) with modifications using [3H]PAF (Platelet Activating Factor, 1-O-Hexadecyl-[Acetyl-3H(N)]-, Hexadecyl PAF) as a substrate. Unlabeled PAF (1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phophorylcholine) was purchased from Enzo Life Sciences, 1-O-Hexadecyl-2-O-[Acetyl-3H(N)]-, Hexadecyl PAF, [acetyl-3H]-, (250µCi (9.25MBq) was purchased from Perkin Elmer, and Bio-Safe II was purchased from Research Products International Corp., Mount Prospect, IL.
He S., Chousterman B.G., Fenn A., Anzai A., Nairz M., Brandt M., Hilgendorf I., Sun Y., Ye Y.X., Iwamoto Y., Tricot B., Weissleder R., Macphee C., Libby P., Nahrendorf M, & Swirski F.K. (2015). Lp-PLA2 Antagonizes Left Ventricular Healing After Myocardial Infarction by Impairing the Appearance of Reparative Macrophages. Circulation. Heart Failure, 8(5), 980-987.
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