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Luck 100

Manufactured by GoldBio
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Sourced in United States

The LUCK-100 is a high-precision laboratory instrument designed for accurate and reproducible measurements. It features a compact design, intuitive user interface, and robust construction. The core function of the LUCK-100 is to provide reliable and consistent data collection in a variety of laboratory settings.

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Lab products found in correlation

Ivis luminar xr imaging system, by PerkinElmer (4 mentions) Adhmgcs2 luc, by VectorBuilder (3 mentions) In vivo xtreme imaging system, by Bruker (1 mentions) Nano glo live cell substrate, by Promega (1 mentions) Pgl4.51 luc2 cmv neo, by Promega (1 mentions) Fugene hd, by Promega (1 mentions) M5524, by Merck Group (1 mentions) Ivis imaging system, by PerkinElmer (1 mentions) D luciferin, by GoldBio (1 mentions) Milli q water, by Merck Group (1 mentions)

15 protocols using luck 100

1

Bioluminescence Imaging of Mice

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Mice were weighed, anesthetized using Ketamine/Xylazine (100 mg/kg and 10 mg/kg body weight respectively), and i.p injected with d-luciferin (GoldBio, #LUCK-100) at 0.15 mg/g body weight, followed by immediate bioluminescence in vivo imaging (Bruker In-vivo Xtreme Imaging System).
He X., Zhang Z., Xue J., Wang Y., Zhang S., Wei J., Zhang C., Wang J., Urip B.A., Ngan C.C., Sun J., Li Y., Lu Z., Zhao H., Pei D., Li C.K, & Feng B. (2022). Low-dose AAV-CRISPR-mediated liver-specific knock-in restored hemostasis in neonatal hemophilia B mice with subtle antibody response. Nature Communications, 13, 7275.
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2

HMGCS2 Regulation in Fasting and Keto

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Adenovirus expressing HMGCS2 (−231/+57) luciferase (AdHMGCS2-Luc) was generated using VectorBuilder (Guangzhou, China). AdHMGCS2-Luc (1 × 109 pfu/kg of body weight) was injected into the tail veins of Pak4 LKO or WT mice, followed by fasting for 24 h or KD-feeding for 3 days. Thereafter, mice were injected with 150 mg/kg of firefly d‐luciferin (#LUCK-100, GoldBio, St Louis, MO, USA), anesthetized after 10 min, and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
Shi M.Y., Yu H.C., Han C.Y., Bang I.H., Park H.S., Jang K.Y., Lee S., Son J.B., Kim N.D., Park B.H, & Bae E.J. (2023). p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1. Nature Communications, 14, 4987.
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3

Bioluminescent Tumor Imaging Protocol

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D-Luciferin was reconstituted as per the manufacturer’s protocol (Goldbio, LUCK-100) and administered intraperitoneally (10 μg/g body weight) into isoflurane-anesthetized animals, 12 min prior to imaging. Animals were excluded if no tumors were present.
Zhang Q., Yang L., Liu Y.H., Wilkinson J.E, & Krainer A.R. (2023). Antisense oligonucleotide therapy for H3.3K27M diffuse midline glioma. Science translational medicine, 15(691), eadd8280.
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4

Yeast-based Luminescence Assays

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Yeast lines expressing NanoLuc or firefly luciferase (FFluc) have been created by transforming the wild-type strain with the corresponding plasmids (see table S4) and selecting on zeocin. For light emission experiments, yeast biomass was resuspended in PBS and used for a drop test in 96-well plates with YPD agar. After 30 hours of incubation at 30°C, we performed luminescence assays by applying 30 μl of 100 μM caffeic acid solution in PBS (for autoluminescence systems), 100 μM d-luciferin (LUCK-100, GoldBio) solution in PBS (for firefly luciferase), or Nano-Glo Live Cell substrate (N2011, Promega) solution (for NanoLuc). Plates were imaged in Tecan Spark with an open filter and automatic attenuation at 0.1-s exposure times for 60 min. Data processing was performed using custom Python scripts. Integral signal was quantified by integration along the “time” axis using the composite trapezoidal rule (trapz function from numpy Python package, v1.22.4).
Palkina K.A., Karataeva T.A., Perfilov M.M., Fakhranurova L.I., Markina N.M., Somermeyer L.G., Garcia-Perez E., Vazquez-Vilar M., Rodriguez-Rodriguez M., Vazquez-Vilriales V., Shakhova E.S., Mitiouchkina T., Belozerova O.A., Kovalchuk S.I., Alekberova A., Malyshevskaia A.K., Bugaeva E.N., Guglya E.B., Balakireva A., Sytov N., Bezlikhotnova A., Boldyreva D.I., Babenko V.V., Kondrashov F.A., Choob V.V., Orzaez D., Yampolsky I.V., Mishin A.S, & Sarkisyan K.S. (2024). A hybrid pathway for self-sustained luminescence. Science Advances, 10(10), eadk1992.
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5

Bioluminescent Imaging of Metastatic Breast Cancer

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MDA-231BR cells stably expressing the luciferase gene (pGL4.51[luc2/CMV/Neo], Promega) were generated by chemical transfection (FuGENE HD, Promega) in the presence of G418 (GoldBio). The presence of metastases in mice intracardially injected with MDA-231BR-luc cells was assessed at various time points after tumor cell injection using noninvasive bioluminescence imaging. Briefly, mice received an intraperitoneal injection of 200 μL of 15 mg/mL D-luciferin potassium salt (GoldBio, #LUCK-100; the final dose of 150 mg/kg). Then, the mice were anesthetized in a chamber containing 2% isoflurane and oxygen and positioned for bioluminescent imaging using a Spectral Lago X imaging system. A series of images were acquired over 30 minutes after the D-luciferin injection. Aura software (version 3.2) was used to identify the regions of interest and integrate the total bioluminescence signal in each ROI. Data for each ROI were analyzed using radiance (photons/s/cm2/steradian).
Mander S., Gorman G.S., Coward L.U., Christov K., Green A., Das Gupta T.K, & Yamada T. (2023). The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents. Neuro-Oncology Advances, 5(1), vdad042.
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6

HMGCS2 Regulation in Fasting and Keto

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Adenovirus expressing HMGCS2 (−231/+57) luciferase (AdHMGCS2-Luc) was generated using VectorBuilder (Guangzhou, China). AdHMGCS2-Luc (1 × 109 pfu/kg of body weight) was injected into the tail veins of Pak4 LKO or WT mice, followed by fasting for 24 h or KD-feeding for 3 days. Thereafter, mice were injected with 150 mg/kg of firefly d‐luciferin (#LUCK-100, GoldBio, St Louis, MO, USA), anesthetized after 10 min, and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
Shi M.Y., Yu H.C., Han C.Y., Bang I.H., Park H.S., Jang K.Y., Lee S., Son J.B., Kim N.D., Park B.H, & Bae E.J. (2023). p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1. Nature Communications, 14, 4987.
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7

Adenoviral-mediated Glucagon-Stimulated Bioluminescence

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Seven-week-old male C57BL/6 J mice were intravenously injected with 1 × 109 p.f.u of CRE luciferase adenovirus (db/db male mice, 5 × 1010 p.f.u) from Vector Biolabs. After 3 days, 6 h-fasted mice were injected intraperitoneally with 100 μg/kg glucagon 1 h before imaging. Then, the mice were injected intraperitoneally with 150 mg/kg firefly d‐luciferin (LUCK-100, GoldBio, St Louis, MO, USA). After 10 min, mice were anesthetized and imaged at 15 min using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
Rah S.Y., Joe Y., Park J., Ryter S.W., Park C., Chung H.T, & Kim U.H. (2023). CD38/ADP-ribose/TRPM2-mediated nuclear Ca2+ signaling is essential for hepatic gluconeogenesis in fasting and diabetes. Experimental & Molecular Medicine, 55(7), 1492-1505.
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8

Transformation and Bioluminescence Assay of BY-2 Cells

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Transformations of BY-2 cells were made by agrobacterial strains encoding plasmids pNK3071 or pNK6260 or pNK6269 (Supplementary Table 4), according to the protocol described above. Forty-eight hours post-infiltration, BY-2 cells were supplemented with 150 µl of MS medium (M5524, Sigma-Aldrich; pH 5.7), containing 100 µM d-Ln (LUCK-100, GoldBio)—in the case of FFLuc, or 0.75 µl of substrate N113 (kit N1110, Promega) in the case of NanoLuc, or no substrate in the case of FBP3. Plates were imaged in Tecan Spark with an open filter and automatic attenuation at 0.1 s exposure times for 30 min. Data processing was performed using custom Python scripts. Integral signal was quantified by integration along the ‘time’ axis using the composite trapezoidal rule (trapz function from numpy Python package, v1.23.5).
Shakhova E.S., Karataeva T.A., Markina N.M., Mitiouchkina T., Palkina K.A., Perfilov M.M., Wood M.G., Hoang T.T., Hall M.P., Fakhranurova L.I., Alekberova A.E., Malyshevskaia A.K., Gorbachev D.A., Bugaeva E.N., Pletneva L.K., Babenko V.V., Boldyreva D.I., Gorokhovatsky A.Y., Balakireva A.V., Gao F., Choob V.V., Encell L.P., Wood K.V., Yampolsky I.V., Sarkisyan K.S, & Mishin A.S. (2024). An improved pathway for autonomous bioluminescence imaging in eukaryotes. Nature Methods, 21(3), 406-410.
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9

Quantifying Muscular Immunization Response

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Mice were imaged at various times after immunization. To quantify luciferase expression, luciferin (GoldBio, Catalog # LUCK-100; weight of 10 μL/g) was administered intraperitoneally 15 min before imaging. Mice were anesthetized and imaged for 45 s using a SII Lago IVIS Imager (Spectral Instruments Imaging). Region of interest (ROI) bioluminescence was used to quantify signal. Each leg (quadriceps) was treated as an individual site of immunization (or individual data point).
Dangi T., Sanchez S., Lew M.H., Awakoaiye B., Visvabharathy L., Richner J.M., Koralnik I.J, & Penaloza-MacMaster P. (2023). Pre-existing immunity modulates responses to mRNA boosters. Cell Reports, 42(3), 112167.
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10

HMGCS2 Regulation in Fasting and Keto

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Adenovirus expressing HMGCS2 (−231/+57) luciferase (AdHMGCS2-Luc) was generated using VectorBuilder (Guangzhou, China). AdHMGCS2-Luc (1 × 109 pfu/kg of body weight) was injected into the tail veins of Pak4 LKO or WT mice, followed by fasting for 24 h or KD-feeding for 3 days. Thereafter, mice were injected with 150 mg/kg of firefly d‐luciferin (#LUCK-100, GoldBio, St Louis, MO, USA), anesthetized after 10 min, and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
Shi M.Y., Yu H.C., Han C.Y., Bang I.H., Park H.S., Jang K.Y., Lee S., Son J.B., Kim N.D., Park B.H, & Bae E.J. (2023). p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1. Nature Communications, 14, 4987.
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