Cd43 bv421

Manufactured by BD

CD43-BV421 is a fluorescent-labeled antibody used in flow cytometry applications to detect and analyze the expression of the CD43 surface protein on cells. It provides a tool for researchers to study the presence and levels of CD43 in various cell types.

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Lab products found in correlation

Cd73 pe cy7, by BioLegend (1 mentions) Lsrii analyzer, by BD (1 mentions) Cd31 pe, by Miltenyi Biotec (1 mentions) Cd45 bv605, by BioLegend (1 mentions) Cd14 fitc, by Thermo Fisher Scientific (1 mentions) Cd235a pe, by Thermo Fisher Scientific (1 mentions) Cd117 pecy7, by BioLegend (1 mentions) Cd127 fitc, by Thermo Fisher Scientific (1 mentions) Tryple, by Thermo Fisher Scientific (1 mentions) Cd34 pe, by BD (1 mentions) Celesta, by BD (1 mentions) Cd31 pe cy7, by BD (1 mentions) Cd34 apc, by BD (1 mentions) Cd45 bv605, by BD (1 mentions) Cd90 apc, by BioLegend (1 mentions) Cd45 apc cy7, by BioLegend (1 mentions)

2 protocols using cd43 bv421

Multiparametric Flow Cytometry Immunophenotyping

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Adherent cells or EBs were dissociated to form a single-cell suspension by TrypLE (Life Technologies) treatment, and washed with FACS buffer (1% FBS and 1 mM EDTA in PBS). The dissociated cells were resuspended in FACS buffer, and labeled with fluorochrome-conjugated anti-human CD34-APC (clone# AC136, Miltenyi Biotec), CD31-PE (clone# AC114.5, Miltenyi Biotec), CD144-Alexa Fluor 700 (clone# 16B1, eBioscience), CD45-BV605 (clone# HI30, BioLegend), CD41-APC/Cy7 (clone# HIP8, BioLegened), CD235a-PE (clone# HIR2, eBioscience), CD43-BV421 (clone# 1G10, BD Biosciences), CD73-PE/Cy7 (clone# AD2, BioLegend), CD117-PE/Cy7 (clone# 104D2, BioLegend), CD127-FITC (clone# eBioRDR5, eBioscience), CD14-FITC (clone# 61D3, eBioscience), or KDR-PerCP (clone# HKDR-1, BioLegend). Flow cytometry was performed on LSR II analyzer (BD Biosciences). Data analysis was performed using FlowJo software or FCS Express software.

BAI H., LIU Y., XIE Y., HOYLE D.L., BRODSKY R.A., CHENG L., CHENG T, & WANG Z.Z. (2015). Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells. Journal of cellular physiology, 231(5), 1065-1076.

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Comprehensive Immunophenotyping of Human Cells

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The following antibodies were used (all anti-human): CD144-Percp-Cy5.5 (Biolegend), CD90-APC (Biolegend), CD31-PE-Cy7 (BD Biosciences), CD34-APC (BD Biosciences), CD34-PE (BD Bioscience), CD43-BV421 (BD Biosciences), CD45-APC-Cy7 (Biolegend), and CD45-BV605 (BD Bioscience). Samples were analyzed on Celesta or LSRII (BD Biosciences). Data from flow cytometry were analyzed using FlowJo software (TreeStar, Ashland, OR).

Shim S.H., Tufa D., Woods R., George T.D., Shank T., Yingst A., Lake J., Cobb L., Jones D., Jones K, & Verneris M.R. (2022). SAHA Enhances Differentiation of CD34+CD45+ Hematopoietic Stem and Progenitor Cells from Pluripotent Stem Cells Concomitant with an Increase in Hemogenic Endothelium. Stem Cells Translational Medicine, 11(5), 513-526.

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