Anti collagen type 2 antibody

Samples were cut into 6 μm sections after being fixed in paraformaldehyde and embedded in paraffin. Hematoxylin and eosin (H&E), safranin-O, Masson's trichrome, and Von Kossa staining were performed according to standard procedures. Two-step indirect immunohistochemical staining was performed to investigate the expression of type I, type II, and type X collagen in matrices using a primary anti-collagen type I antibody (mouse anti-rabbit, 1 : 50; Abcam, Cambridge, MA, USA), anti-collagen type II antibody (mouse anti-rabbit, 1 : 50; Acris, Herford, Germany), and anti-collagen type X antibody (mouse anti-rabbit, 1 : 50; Abcam, Cambridge, MA, USA), followed by a horseradish peroxidase-conjugated anti-mouse antibody (1 : 200 in PBS; Santa Cruz, Dallas, Texas, USA) and color development with diaminobenzidine tetrahydrochloride (Santa Cruz, Texas, USA). The sections were counterstained with hematoxylin solution (Mayer's).

Samples were fixed in 4% paraformaldehyde and embedded in paraffin and then cut into 8 um sections. Sections were stained with hematoxylin and eosin (H & E), safranin-O, Masson trichrome or Von Kossa. To investigate the expression of type I, type II and type X collagen in matrices and vascular endothelial growth factor (VEGF), some sections were processed for two-step indirect immunohistochemical staining as studied previously [17 (link)]. Briefly, expression of type I collagen, type II collagen, type X collagen and VEGF was detected using primary anti-collagen type I antibody (mouse anti-rabbit, 1:50; Abcam, Cambridge, MA USA), anti-collagen type II antibody (mouse anti-rabbit, 1:50; Acris, Herford Germany), anti-collagen type X antibody (mouse anti-rabbit, 1:50; Abcam) and anti-VEGF antibody (mouse anti-rabbit, 1:50; Abcam), followed by horseradish peroxidase-conjugated anti-mouse antibody (1:200 in PBS; Santa Cruz Dallas, Texas, USA) and color development with diaminobenzidine tetrahydrochloride (Santa Cruz).