T47D (ATCC® HTB-133™), MCF7 (ATCC® HTB-22™), MDA-MB-231 (ATCC® CRM-HTB-26™), MDA-MB-468 (ATCC® HTB-132™) and BT-549 (ATCC® HTB-122™) human BC cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). T47D, MCF7, MDA-MB-231 and MDA-MB-468 cancer cells were grown in DMEM/Nutrient F-12 Ham (DMEM/F12) culture medium (Sigma) with 10% FBS (Sigma), penicillin (100 IU/mL) (Sigma) and streptomycin (100 μg/mL) (Sigma). BT-549 cells were maintained in Roswell Park Memorial Institute Medium (RPMI) culture medium (ATTC) with 0.023 U/mL insulin (Sigma), 10% FBS (ATTC), penicillin (100 IU/mL) (Sigma) and streptomycin (100 μg/mL) (Sigma). Mycoplasma-free cultures were maintained in a humidified atmosphere with 5% CO2 at 37°C.
Dmem nutrient f 12 ham dmem f12 culture medium
Manufactured by Merck Group
DMEM/Nutrient F-12 Ham (DMEM/F12) is a cell culture medium composed of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. It is designed to support the growth and maintenance of a variety of cell types, including those from human, animal, and plant sources.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 468, by Merck Group (2 mentions)
Mcf 7, by Merck Group (2 mentions)
Mda mb 231, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Insulin, by Merck Group (1 mentions)
T47d atcc htb 133, by American Type Culture Collection (1 mentions)
Bt 549 atcc htb 122, by American Type Culture Collection (1 mentions)
2 protocols using dmem nutrient f 12 ham dmem f12 culture medium
1
Culturing Human Breast Cancer Cell Lines
2
Culturing Human Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MCF7 (ATCC® HTB-22TM), T47D (ATCC® HTB-133TM), MDA-MB-231 (ATCC® CRM-HTB-26TM), and MDA-MB-468 (ATCC® HTB-132TM) human BC cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). T47D, MCF7, MDA-MB-231, and MDA-MB-468 cancer cells were grown in DMEM/Nutrient F-12 Ham (DMEM/F12) culture medium (Sigma) with 10% FBS (Sigma), penicillin (100 μg/mL), (Sigma) and streptomycin (100 μg/mL) (Sigma). Mycoplasma-free cultures were maintained in a humidified atmosphere with 5% CO2 at 37 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!