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Human genomic dna library

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The Human Genomic DNA Library is a collection of DNA fragments representing the entire human genome. It is designed for use in various molecular biology applications, such as gene cloning, sequencing, and genomic analysis. The library is created by randomly fragmenting human genomic DNA and inserting the fragments into suitable vectors, allowing for the preservation and study of the complete human genetic information.

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Human genomic DNA (79 citations)

2 protocols using human genomic dna library

1

Cloning CD44v8-10 Reporter Construct

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A reporter construct was generated using standard cloning techniques. The CD44 genomic DNA, including CD44v8-10 exon and flanking introns (500 bp each) was PCR amplified from the Human Genomic DNA Library (Promega, Madison, WI, USA). The PCR product was cloned into MluI and BamHI enzyme sites in pFlare9A-Dup34 vector28 (link).
Zhang P., Feng S., Liu G., Wang H., Fu A., Zhu H., Ren Q., Wang B., Xu X., Bai H, & Dong C. (2016). CD82 suppresses CD44 alternative splicing-dependent melanoma metastasis by mediating U2AF2 ubiquitination and degradation. Oncogene, 35(38), 5056-5069.
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2

Cloning CD44v8-10 Reporter Construct

Check if the same lab product or an alternative is used in the 5 most similar protocols
A reporter construct was generated using standard cloning techniques. The CD44 genomic DNA, including CD44v8-10 exon and flanking introns (500 bp each) was PCR amplified from the Human Genomic DNA Library (Promega, Madison, WI, USA). The PCR product was cloned into MluI and BamHI enzyme sites in pFlare9A-Dup34 vector28 (link).
Zhang P., Feng S., Liu G., Wang H., Fu A., Zhu H., Ren Q., Wang B., Xu X., Bai H, & Dong C. (2016). CD82 suppresses CD44 alternative splicing-dependent melanoma metastasis by mediating U2AF2 ubiquitination and degradation. Oncogene, 35(38), 5056-5069.
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