Curcumin [(1E,6E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] and DSPC (1,2-Distearoyl-sn-glycerol-3-phosphocholine were purchased from Sigma Chemicals, India. Dimethyl sulphoxide, Trypan blue, and methylthiazoly-diphenyl-tetrazolium bromide (MTT) were procured from Himedia, India. Dialysis membrane, Dulbecco's Modified Eagle Medium (DMEM), Antibiotics-antimycotics mixture, and fetal bovine serum (FBS) were purchased from GibCo, India.
Dimethyl sulphoxide
Manufactured by HiMedia
Sourced in India
Dimethyl sulphoxide (DMSO) is a colorless, odorless, and polar aprotic solvent with a wide range of applications in various industries, including pharmaceuticals, biomedical research, and chemical synthesis. It has a unique ability to penetrate biological membranes and facilitate the transport of other compounds. DMSO is commonly used as a solvent, cryoprotectant, and carrier for various active ingredients.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by HiMedia (2 mentions)
Methanol, by HiMedia (2 mentions)
Glacial acetic acid, by HiMedia (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Antibiotics antimycotics mixture, by Thermo Fisher Scientific (1 mentions)
Trypan blue, by HiMedia (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Curcumin, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by HiMedia (1 mentions)
Trypsin edta solution, by HiMedia (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by HiMedia (1 mentions)
Dnase, by HiMedia (1 mentions)
Rnase, by HiMedia (1 mentions)
Antibiotic antimycotic solution, by HiMedia (1 mentions)
Dulbecco s modified eagle s medium dmem, by HiMedia (1 mentions)
Escherichia coli, by Microbial Type Culture Collection (1 mentions)
Nutrient agar, by HiMedia (1 mentions)
Luria bertani broth, by HiMedia (1 mentions)
Sodium dodecyl sulfate (sds), by HiMedia (1 mentions)
8 protocols using dimethyl sulphoxide
1
Curcumin-DSPC Nanoformulation Preparation
2
Silk Cocoon-Derived Biomaterials for Cell Culture
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Silk cocoons were obtained from the Government Silk Cocoon market, Vellore, India. DNase, RNase, phosphate buffered saline, sodium dodecyl sulfate, Dulbecco’s modified eagle’s medium (DMEM), trypsin—EDTA solution, fetal bovine serum, antibiotic antimycotic solution, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, dimethyl sulphoxide, Luria bertani broth, nutrient agar, and formalin, were purchased from HiMedia Laboratories Private Limited, India. Escherichia coli was procured from Microbial Type Culture Collection, Institute of Microbial Technology, Chandigarh, India.
3
Ciprofloxacin-Chitosan Nanoparticle Formulation
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Ciprofloxacin was obtained from Himedia, Mumbai, (India), chitosan, sodium triphosphate pentabasic (TPP), fluorescein isothiocyanate (FITC) dye, and phorbol myristate acetate (PMA). Glacial acetic acid; NaOH; dimethyl sulphoxide(DMSO); trehalose; phosphate buffer saline (PBS); syringe filter (0.22 μ pore size);and RPMI culture media were obtained from Himedia, Mumbai (India).
4
Biochemical Reagents for Cell Assays
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Classical media (Dulbecco’s modified essential medium), dimethyl sulphoxide (DMSO), fetal bovine serum (FBS), L-nitro-arginine methyl ester (L-NAME) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), RIPA Buffer (10X), sulphuric acid, chloroform liquid ammonium hydroxide, and methanol was procured from HiMedia Laboratories (Mumbai, Maharashtra, India). Vasicine was purchased from Natural remedies (Bangalore, Karnataka, India). Sulfanilamide and naphthylethylenediamine were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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Classical media (Dulbecco’s modified essential medium), dimethyl sulphoxide (DMSO), fetal bovine serum (FBS), L-nitro-arginine methyl ester (L-NAME) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), RIPA Buffer (10X), sulphuric acid, chloroform liquid ammonium hydroxide, and methanol was procured from HiMedia Laboratories (Mumbai, Maharashtra, India). Vasicine was purchased from Natural remedies (Bangalore, Karnataka, India). Sulfanilamide and naphthylethylenediamine were purchased from Sigma-Aldrich (St. Louis, MO, USA).
5
Antioxidant Assay Protocol for Natural Products
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2,2- Azinobis-3-ethylbenzothiazoline-6-sulphonic acid disodium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Dimethyl Sulphoxide, Sodium acetate trihydrate ACS, Ferric chloride hexahydrate A.R., Ferrous sulphate heptahydrate A.R., Folin ciocalteu’s reagent L.R., Gallic acid monohydrate, L-Ascorbic acid A.R., Acetic acid glacial A.R., Sodium carbonate ACS, Potassium persulphate A.R., were purchased from Hi-media, Mumbai, India. 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox), Aluminium chloride AR, and Quercetin ≥95% (HPLC) solid were purchased from Sigma-Aldrich, USA. Acetonitrile, methanol (LC-MS grade) and formic acid (analytical grade) were purchased from Fluka, Sigma-Aldrich (St. Louis, MO, USA). Ultra pure water was obtained from a Direct-Q 8 UV water purification system (EMD Millipore Corporation, Billerica, MA, USA). All other reagents including solvents were of analytical grade and were procured from Hi-Media, Mumbai, India.
6
Antibiotic Resistance Prevalence Study
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Using an approximate prevalence rate of 50%, confidence interval of 95%, precision of 5% and using the formula n = (Z1-α)2(P (1 − P)/D2), a sample size of 385 was calculated. It was rounded off to 400 samples per center including 200 of E. coli and 200 of K. pneumoniae. The power of study was set at 80%. Categorical data was analyzed by Chi-square test whereas z test for proportion was used to determine the relationship between groups. P ≤ 0.05 was considered to be statistically significant.
Culture media, antibiotic disks, APB acid, dimethylsulphoxide, ATCC strains were procured from Hi-Media laboratories, Mumbai, India. Pure antibiotic powders for MIC determination were procured from Sigma-Aldrich, Bangalore, India.
Culture media, antibiotic disks, APB acid, dimethylsulphoxide, ATCC strains were procured from Hi-Media laboratories, Mumbai, India. Pure antibiotic powders for MIC determination were procured from Sigma-Aldrich, Bangalore, India.
7
Mung Bean-Mediated Gold Nanoparticles
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Mung (Vigna radiata) bean seed was obtained from local market, Tetrachloroauric acid [HAuCl4], Dulbecco’s Modified Eagle Medium [DMEM], Fetal Bovine Serum, Trypsin, purchased from Himedia, Dimethylsulphoxide [DMSO], Crystal violet, Trypan blue, Ethidium Bromide, Acridine orange, MTT, Alamar Blue, Phosphate buffered saline [PBS], Methanol etc ere obtained from Thermo scientific.
8
Quantification of Flavonoid Compounds
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Standards of flavonoid compounds, such as hesperidin, narirutin, naringin, quercitin, and naringenin (97% purity), antioxidant standard Trolox (97% purity), radical cation ABTS+ azino-bis [3-ethylbenzthiazoline-6-sulfonic acid], 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 4, 6-Tri (2-pyridyl)-s-triazine (TPTZ), and gallic acid were purchased from Sigma–Aldrich (Mumbai, India). Chemicals such as sodium acetate trihydrate, ammonium acetate, acetonitrile, dimethyl sulphoxide, and acetonitrile were of the HPLC grade for use in the extraction procedure and in HPLC technique (Himedia, India). Additional chemical substances and reagents utilised in the study, such as methanol, ferric chloride, folin-Ciocalteu reagent, sodium carbonate, manganese dioxide, and 37% hydrochloric acid, were of analytical grade (Himedia, India).
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