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Strata x polymeric reverse phase column

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Strata-X polymeric reverse phase columns are solid-phase extraction (SPE) columns designed for the purification and concentration of analytes in various sample matrices. The columns utilize a polymeric sorbent material for retention of analytes based on reversed-phase interactions. The core function of these columns is to facilitate the extraction, cleanup, and concentration of target compounds from complex samples prior to instrumental analysis.

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Lab products found in correlation

Ultraflextreme maldi tof tof instrument, by Bruker (3 mentions) C1000 thermal cycler, by Bio-Rad (3 mentions) Jupiter proteo c 12 column, by Phenomenex (2 mentions) Acquity uplc system, by Waters Corporation (2 mentions) Cp chirasil l val fused silica column, by Agilent Technologies (2 mentions) Agilent 7890 gas chromatograph, by Agilent Technologies (2 mentions) Bca protein assay, by Thermo Fisher Scientific (1 mentions) Xl 2000, by Bioventus (1 mentions) Delta 600, by Waters Corporation (1 mentions) Voyager de str instrument, by Thermo Fisher Scientific (1 mentions) Synergy h4 microplate reader, by Agilent Technologies (1 mentions)

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Strata-X (72 citations) Strata-X columns (17 citations) Strata-X 33 um polymeric reverse phase column (4 citations)

5 protocols using strata x polymeric reverse phase column

1

Protein Extraction and Trypsin Digestion Protocol

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Each tissue sample was suspended in 1.2 ml lysis buffer (6M Guanadine HCl, 100mM Tris pH 8) before being probe sonicated (Misonix XL-2000) to lyse the cells. After sonication a protein BCA assay (Thermo Scientific) was performed to determine protein concentrations of the lysate. Sample lysates ranged in concentration from approximately 6–13 mg/ml. 500ug of protein was aliquoted from each sample lysate. Each aliquot was brought up to 90% methanol before being centrifuged at 14,000 g for 5 min. Supernate was disposed and the precipitate was resuspended in 240ul of reducing and alkylating buffer (8M urea, 10mM TCEP, 40mM CAA, 100mM Tris pH 8). The sample solution was then diluted to 25% concentration with 100mM Tris, pH 8. Trypsin was added to the protein lysate sample at a ratio of 50:1 w/v and digested overnight. Digested samples were desalted using Strata-X Polymeric Reverse Phase column (Phenomenex). Samples were then dried in SpeedVac Concentrator.
McKetney J., Runde R., Hebert A.S., Salamat S., Roy S, & Coon J.J. (2019). Proteomic Atlas of the Human Brain in Alzheimer’s Disease. Journal of proteome research, 18(3), 1380-1391.
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2

Protein Extraction and Digestion Protocol

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Samples were brought to 90% methanol and centrifuged, with the precipitate pellet then resuspended in 8M urea, 10mM TCEP, 40mM CAA, 100mM Tris pH 8. The solution was then diluted to 25% concentration with 100mM Tris, pH 8, and trypsin was added at a ratio of 50:1 w/w and digested overnight at ambient temperature. Digested peptides were desalted using Strata-X Polymeric Reverse Phase column (Phenomenex).
McKetney J., Panyard D.J., Johnson S.C., Carlsson C.M., Engelman C.D, & Coon J.J. (2021). Pilot Proteomic Analysis of Cerebrospinal Fluid in Alzheimer’s Disease. Proteomics. Clinical applications, 15(2-3), e2000072.
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3

Multimodal Analytical Techniques for Comprehensive Characterization

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All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). DNA sequencing was performed by ACGT, Inc. MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker). LC-ESI-Q/TOF MS and MS/MS analyses were conducted using a Synapt G1 instrument with an Acquity UPLC system (Waters), which was equipped with a Jupiter Proteo C12 column (5 µm; 90 Å; 100 × 1.0 mm) (Phenomenex). GC-MS analysis was performed in the Mass Spectrometry Laboratory (School of Chemical Sciences, UIUC) on an Agilent 7890 gas chromatograph (Agilent) with a CP-Chirasil-L-Val fused silica column (25 m × 0.25 mm × 0.15 µm) (Agilent). Solid phase extraction was performed with Strata-X polymeric reverse phase columns (Phenomenex).
Tang W., Jiménez-Osés G., Houk K.N, & van der Donk W.A. (2014). Substrate Control in Stereoselective Lanthionine Biosynthesis. Nature chemistry, 7(1), 57-64.
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4

Multimodal Analytical Techniques for Comprehensive Characterization

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All polymerase chain reactions (PCR) were carried out on a C1000™ thermal cycler (Bio-Rad). DNA sequencing was performed by ACGT, Inc. MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker). LC-ESI-Q/TOF MS and MS/MS analyses were conducted using a Synapt G1 instrument with an Acquity UPLC system (Waters), which was equipped with a Jupiter Proteo C12 column (5 µm; 90 Å; 100 × 1.0 mm) (Phenomenex). GC-MS analysis was performed in the Mass Spectrometry Laboratory (School of Chemical Sciences, UIUC) on an Agilent 7890 gas chromatograph (Agilent) with a CP-Chirasil-L-Val fused silica column (25 m × 0.25 mm × 0.15 µm) (Agilent). Solid phase extraction was performed with Strata-X polymeric reverse phase columns (Phenomenex).
Tang W., Jiménez-Osés G., Houk K.N, & van der Donk W.A. (2014). Substrate Control in Stereoselective Lanthionine Biosynthesis. Nature chemistry, 7(1), 57-64.
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5

Analytical Methods for Biomolecule Characterization

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All polymerase chain reactions (PCRs) were carried out on a C1000™ thermal cycler (Bio-Rad). DNA sequencing was performed by ACGT, Inc. Preparative HPLC was performed using a Waters Delta 600 instrument equipped with appropriate columns. Solid phase extraction was performed with Strata X polymeric reverse phase columns (Phenomenex). MALDI-TOF MS was carried out on a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF instrument (Bruker) or a Voyager-DE-STR instrument (Applied Biosystems). The detection of peptides with low molecular weights (700–3,500 Da), peptides with medium molecular weights (5,000–20,000 Da) and proteins with high molecular weights (20,000–50,000 Da) was achieved by using different instrument settings optimized for these mass ranges. LC-ESI-Q/TOF MS analyses were conducted using a Micromass Q-Tof Ultima instrument (Waters) equipped with a Vydac C18 column (5 μm; 100 A; 250 × 1.0 mm). Absorbance of rabbit hemoglobin solution was measured in 96-well plates with a Synergy™ H4 Microplate Reader (BioTek). Negative numbers are used for amino acids in the leader peptide counting backwards from the leader peptide cleavage site.
Tang W., Bobeica S.C., Wang L, & van der Donk W.A. (2018). CylA is a Sequence-Specific Protease Involved in Toxin Biosynthesis. Journal of industrial microbiology & biotechnology, 46(3-4), 537-549.
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