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Spectra por molecular porous membrane tubing

Manufactured by Repligen
Sourced in United States

Spectra/Por® molecular porous membrane tubing is a laboratory product designed for various applications. It is a semi-permeable membrane tubing that allows the passage of molecules based on their size and weight. The core function of this tubing is to facilitate the separation, concentration, and purification of molecules in various research and industrial processes.

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2 protocols using spectra por molecular porous membrane tubing

1

Optimizing Transdermal Liposomal Delivery

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Phospholipon 90G (soybean phosphatidylcholine) (P90G) was kindly donated by Lipoid (Steinhausen, Switzerland). Cholesterol and B12 (purity 95%) were purchased from Acofarma (Madrid, Spain). Tween 80, phosphate salts, and sodium chloride to prepare buffers were from Scharlab (Sentmenat, Spain). Regenerated cellulose dialysis membranes with a cut-off of 14kDa (Spectra/Por® molecular porous membrane tubing) for the in vitro release studies were acquired from Repligen (Waltham, MA, USA). HPLC-quality water obtained by Milli-Q purification (Millipore, Madrid, Spain) with resistance > 18 MΩ cm and TOC < 10 ppb was used for liposome reconstitution and HPLC analysis. Methanol for the HPLC mobile phase was purchased at VWR International (Radnor, PA, USA). For the tape-stripping studies, adhesive tape Tesafilm kristall-klar (33 mm × 19 mm, cut to 30 mm × 19 mm sections) was obtained from Tesa (Hamburg, Germany); Parafilm Sealing Film—50 mm (2”) Film Width × Roll Length 75 m (Parafilm, Madrid, Spain) was used; glass beads were obtained from MERCK (Madrid, Spain). Pig ears were procured from a local slaughterhouse (Mercavalencia, Valencia, Spain).
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2

Corneal Drug Delivery Protocol

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CPXb was bought from Acros Organics (New Jersey, USA), and CPXs was acquired from Cayman Chemical Company (Michigan, USA). Soluplus was kindly donated by BASF SE (Ludwigshafen, Germany). Poly-vinyl alcohol (PVA), polycaprolactone (PCL), gelatin from bovine skin, and chitosan were purchased from Sigma-Aldrich (Missouri, USA), and propylene glycol (PG), polyethylene glycol (PEG), hydroxy ethyl cellulose (HEC), and hydroxy propyl methyl cellulose (HPMC) were purchased from Fagron Iberica (Barcelona, Spain). Organic solvents such as DMSO and chloroform were purchased from Sigma-Aldrich (Missouri, USA). For the phosphate-buffered saline (PBS) used, sodium chloride, potassium chloride, sodium phosphate dibasic, and potassium phosphate monobasic were purchased from Scharlab (Barcelona, Spain). Ultrapure water used throughout the studies was obtained from a Q-POD dispenser (Merck Millipore, Burlington, MA, USA). Acetonitrile for the HPLC mobile phase was purchased VWR International (Radnor, PA, USA). Dialysis membranes (Spectra/Por molecular porous membrane tubing) for in vitro release studies were acquired from Repligen (MA, USA). Corneas were from porcine eyes procured from a local slaughterhouse Mercavalencia (Valencia, Spain).
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