Rabbit anti eap30

The following primary antibodies were used: mouse anti-ß-Actin and mouse anti-FLAG (Sigma-Aldrich, St. Louis, MO, USA); mouse anti-Alix, mouse anti-EAP20, rabbit anti-EAP30, rabbit anti-EAP45, and mouse anti-Tsg101 (Santa Cruz Biotechnology, Dallas, TX, USA); mouse anti-GFP (BD Biosciences, Heidelberg, Germany); mouse anti-HA (Covance, Princeton, NJ, USA); and rabbit anti-Nedd4 (Cell Signaling, Danvers, MA, USA). Peroxidase-labeled, secondary antibodies were obtained from Dianova (Hamburg, Germany), and fluorophore-labeled antibodies were purchased from Molecular Probes (Eugene, OR, USA).

For immunoprecipitation of HBV virions, a mixture of rabbit antibodies against the L and S envelope proteins was used as described [26] (link). Polyclonal antisera against recombinant native (K45) or denatured (K46) core particles were raised in rabbits [33] (link). The MA18/7 mouse antibody recognizing the HBV L protein was a gift from K.-H. Heermann (University of Göttingen, Germany). Commercially available antibodies were as follows: mouse anti-β-actin (Sigma-Aldrich), rabbit anti-CD63 (Santa Cruz Biotechnology), mouse anti-EAP20 (VPS25, Santa Cruz Biotechnology), rabbit anti-EAP30 (Santa Cruz Biotechnology), rabbit anti-EAP45 (Santa Cruz Biotechnology), mouse anti-FLAG (Sigma-Aldrich), mouse anti-Tsg101 (Santa Cruz Biotechnology), and rabbit anti-Vps28 (Santa Cruz Biotechnology). Peroxidase-labeled, secondary antibodies were obtained from Dianova, and fluorophor-labeled antibodies were purchased from Molecular Probes.