Donkey anti rabbit and sheep anti mouse

Influenza A virus NS1 antibody, a gift from J.A. Richt (National Animal Disease Center, Iowa)^{28 (link)} was used at a 1:10000 dilution. β-actin monoclonal antibody (Sigma-Aldrich, clone AC-74, A5528) was used at a 1:5000 dilution. His tag monoclonal antibody (TAKARA & Clontech, 631210) was used at a 1:5000 dilution. ANTI-FLAG® M2 monoclonal antibody (Sigma-Aldrich, F1804) was used at a 1:1000 dilution. Horseradish peroxidase (Hrp)-conjugated secondary antibodies included donkey anti-rabbit and sheep anti-mouse (GE Healthcare, NA934V and NA931V, respectively). Polyclonal rabbit anti-Nup98 antibody^{29 (link)} was used at a 1:1000 dilution. Mouse anti-nuclear pore complex proteins antibody MAB414 (Abcam, ab24609) was used at a 1:1000 dilution. Mouse anti-NXF1 antibody (Sigma-Aldrich, T1076–200UL) was used at a 1:2000 dilution. Mouse anti-M2 antibody (Thermo Fisher Scientific, MA1–082) was used at a 1:1000 dilution. Rabbit anti-HA antibody (Genetex, GTX127357) was used at a 1:1000 dilution. Rabbit anti-NA antibody (Genetex, GTX125974) was used at a 1:1000 dilution. Goat anti-influenza A virions antibody (Meridian Life Science, B65141G) was used at a 1:1000 dilution. Mouse IgG (Thermo Fisher Scientific, 31903) was diluted to 0.4 mg/ml.