Anti human cd24 pe

Anti-Human/Mouse CD44 FITC (eBioscience) and Anti-Human CD24 PErCP-eFluor 710 (eBioscience), Anti-Human CD24 PE (eBioscience) and Anti-Human CD326 (EpCAM) PerCP-eFluor 710 (eBioscience) were used for flow cytometric analysis. Cells were harvested by trypsinization and washed once in cold PBS. Next, cells were counted and resuspended in cold PBS at a concentration of 1 × 10⁶ cells per 100 μl. Staining was performed by incubating 100 μl cells with 5 μl antibody on ice for 30 min.

The expression levels of Ki-67, CD44 (Cluster of differentiation 44) and CD24 (Cluster of differentiation 24) were analyzed by flow cytometry.
Cells were dissociated using Trypsin Solution without EDTA (Beyotime, Shanghai, China) and resuspended in Flow Cytometry Staining buffer (MULTI SCIENCES, Hangzhou, China). Cells were stained with anti-human Ki-67 (BioLegend, USA) after permeabilization with 70% ethanol at −20 °C for 1 h. Data were compared by the mean of log fluorescence intensity. Cells were incubated with anti-human CD44-APC, anti-human CD24-PE and the APC and PE isotype control antibodies (eBioscience, USA) for 30 min at room temperature. Cells were washed and evaluated by flow cytometry (BD Biosciences, USA) to determinate population distribution.