Clone pc 61

Manufactured by BioXCell

Sourced in United States

The Clone PC-61 is a compact desktop centrifuge designed for general laboratory use. It features a rotor capacity of 6 x 15 mL tubes and a maximum speed of 6,000 RPM. The device is suitable for a variety of common centrifugation applications in biological and medical research settings.

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Anti-CD25 (clone PC-61.5.3, (13 citations) PC-61.5.3 (10 citations) Anti-CD25 monoclonal antibody (clone PC-61.5.3) (2 citations)

5 protocols using clone pc 61

Anti-CD25 Treatment in Ovalbumin-Immunized Mice

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B6 and HOD recipient mice were treated weekly with an intraperitoneal (i.p.) injection of 300 μg of anti-CD25 (BioXcell, clone PC-61) or anti-HRPN Rat IgG1 isotype control (BioXcell). In some experiments, recipient mice were immunized subcutaneously with 100 μg of ovalbumin (Sigma) emulsified in CFA (Difco Labs). Sera were collected 14 days post-treatment.

Richards A.L., Kapp L.M., Wang X., Howie H.L, & Hudson K.E. (2016). Regulatory T Cells Are Dispensable for Tolerance to RBC Antigens. Frontiers in Immunology, 7, 348.

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In vivo CD25+ Cell Depletion

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CD25⁺ cells Deplation in vivo was performed as previously described [12 (link)]. Briefly, mice received 15 mg/kg of rat anti-mouse CD25 mAb (clone PC-61, BioXcell) on days −2, 0, 2 after transplantation, CD25⁺ cell depletion was confirmed on POD7 by staining splenocytes. Rat IgG1 injections at the same time point to reciprocal recipients served as isotype-matched controls.

Qi H., Chen G., Huang Y., Si Z, & Li J. (2015). Foxp3-modified bone marrow mesenchymal stem cells promotes liver allograft tolerance through the generation of regulatory T cells in rats. Journal of Translational Medicine, 13, 274.

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CD25+ T Cell Depletion Protocol

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Depletion of CD25+ T cells was achieved with an anti-CD25 monoclonal antibody (CD25-mAb; Clone PC61, (BioXCell, West Lebanon, NH, USA)) (Setiady et al., 2010 (link)). Using a single IP injection of the CD25-mAb (IgG used as a control) the morning of I/R surgery, CD25+FoxP3+ T cells (CD4+CD3+) were depleted by about 40% 1-day post injection (data not shown). At the end of the 3-day reperfusion period, we were able to achieve a ~35% depletion of CD25+, FoxP3+ T cells (CD4+, CD3+).

Danilo C.A., Constantopoulos E., McKee L.A., Chen H., Regan J.A., Lipovka Y., Lahtinen S., Stenman L.K., Nguyen T.V., Doyle K.P., Slepian M.J., Khalpey Z.I, & Konhilas J.P. (2017). Bifidobacterium animalis subsp. lactis 420 mitigates the pathological impact of myocardial infarction in the mouse. Beneficial microbes, 8(2), 257-269.

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Immunomodulatory Therapy for Tumor Control

Cited 2 times

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Days indicated are after tumor challenges. Mice were injected with 100 μg of αB7-H1 (clone 10F.9G2), αPD-1 (clone RMP1-14) or αCTLA-4 (clone 9H10, all from BioXCell) monoclonal antibodies intraperitoneally (i.p.) on days 4, 7 and 12. αCD25 (250 μg, clone PC-61.5.3, BioXCell) was administered i.p. on day 2. Denileukin diftitox (DD, Eisai), a fusion protein of diphtheria toxin and human interleukin-2 (IL-2) (18 (link)), that depletes mouse Tregs to treat distinct carcinomas (15 (link),16 (link),19 (link)), was given 5 μg i.p. every 4 days for EL4 challenge, and every 5 days for B16 and ID8agg challenge. Foxp3^DTR mice engineered to allow Treg-specific depletion using diphtheria toxin (15 (link)) were injected i.p. with 1 μg/kg diphtheria toxin every 3 days starting on day 4. Rapamycin (LC Laboratories) or vehicle control (0.25% Tween 80 + 0.25% PEG400) was given i.p. daily for 5 consecutive days/week at indicated doses starting on day 4, for times indicated. Rapamycin at 1–8 mg/kg is defined as typical, and 0.075 mg/kg as low dose (LD).

Liu Y., Pandeswara S., Dao V., Padrón Á., Drerup J.M., Lao S., Liu A., Hurez V, & Curiel T.J. (2016). Biphasic rapamycin effects in lymphoma and carcinoma treatment. Cancer research, 77(2), 520-531.

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Treg Cell Ablation and Depletion Protocols

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For Treg cell ablation studies, DT (Sigma-Aldrich) was injected intraperitoneally (i.p.) at 50 μg per kg of body weight at the indicated times. For neutralization and depletion studies, anti-mouse IFN-γ (1 mg, clone XMG1.2, Bio X Cell), anti-mouse CD11c (500 μg, clone N418, Bio X Cell), anti-mouse CD8 (250 μg, clone 53-6.72, Bio X Cell), anti-mouse CD4 (150 μg, clone GK1.5, Bio X Cell), or anti-mouse CD25 (500 μg, clone PC-61.5.3, Bio X Cell) was injected i.p. at the indicated times. Isotype control antibodies used were rat IgG1, Armenian hamster IgG, or rat IgG2a from Bio X Cell, respectively.

Jang J.E., Hajdu C.H., Liot C., Miller G., Dustin M.L, & Bar-Sagi D. (2017). Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer. Cell Reports, 20(3), 558-571.

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