Goat anti talin c 20

The following antibodies were used: goat anti-talin C-20 (Santa Cruz sc-7534, IF 1/50), mouse anti-vinculin clone hvin-1 (Sigma-Aldrich V9131, IF 1/50), mouse anti-paxillin (BD Biosciences 61005, IF 1/50), mouse anti-cortactin clone 4F11 (p80/85) (Sigma-Aldrich 05–180, IF 1/50), mouse anti-filamin A clone PM6/317 (Sigma-Aldrich MAB1678, IF 1/50), mouse anti-α-actinin1 clone BM-75.2 (Sigma-Aldrich A5044, IF 1/50) and mouse monoclonal antibody against the human LAMP-1 (H4A3, Santa Cruz Biotechnology, IF 1/100).

Antibodies used for western blot analysis: mouse anti-GFP (1:1000; Proteintech, #50430-2-AP), rabbit anti-pFAK Y576 (1:200; Santa Cruz, #sc-16563), rabbit anti-pFAK Y397 (1:200; Novus, #NBPI-60837), rabbit anti-pPaxillin Y31 (1:200; Santa Cruz, #sc-14035), mouse anti-FAK (1:1000; Proteintech, #66258-I-1g), mouse anti-Paxillin (1:5000; BD Biosciences, #610051), rabbit anti-FLAG (1:1000; Proteintech, #80010-1-RR).
Antibodies used in immunofluorescence experiments: mouse anti-FAK 4.47 (1:1000; Millipore, #05-537), mouse anti-FAK (1:1000; Proteintech, #66258-I-1g), rabbit anti-FAK C-20 (1:200; Santa Cruz, #sc-558), rabbit anti-pFAK Y576 (1:200; Santa Cruz, #sc-16563), rabbit anti-pFAK Y397 (1:200; Novus, #NBPI-60837) goat anti-Talin C-20 (1:750; Santa Cruz, #sc-7534), mouse anti-Paxillin (1:750; R&D Systems, #AF4259), mouse anti-Vinculin (1:1000; Sigma, #V9131), rabbit anti-Vinculin (1:2000; Proteintech, #26520-I-AP), mouse anti-av Integrin H-2 (1:100; Santa Cruz, #sc-376156), rabbit anti-pPaxillin Y31 (1:200; Santa Cruz, #sc-14035), mouse anti-p-Tyr (pY20) (1:200; Santa Cruz, #sc-508), rat anti-active β1-Integrin 9EG7 (1:700; BD Bioscences, #550531), mouse anti-p130 Cas 35B.1A4 (1:500; Santa Cruz, #sc-20029), mouse anti-Tensin C-2 (1:1000; Santa Cruz, #sc-376367).