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Anti rpn1

Manufactured by Santa Cruz Biotechnology
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Anti-RPN1 is a laboratory antibody product developed by Santa Cruz Biotechnology. It is designed to detect the RPN1 (Ribophorin I) protein, which is a component of the oligosaccharyltransferase complex involved in N-linked glycosylation. The antibody can be used in various techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the RPN1 protein.

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Lab products found in correlation

Vimentin, by Santa Cruz Biotechnology (1 mentions) Anti pakt thr308, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Anti gsk3β, by ABclonal (1 mentions) Anti gapdh, by Transgene (1 mentions) Anti snail, by Cell Signaling Technology (1 mentions) Endo h, by New England Biolabs (1 mentions) Anti pan akt, by Cell Signaling Technology (1 mentions) Anti p akt ser473, by Cell Signaling Technology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti ha, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Ly294002, by Merck Group (1 mentions) Pngase f, by New England Biolabs (1 mentions) Puromycin, by Solarbio (1 mentions) Anti lc3b, by Merck Group (1 mentions) N acetyl l cysteine, by Fujifilm (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Trehalose, by Merck Group (1 mentions)

2 protocols using anti rpn1

1

Regulation of GSK3β Signaling Pathway

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Antibodies used in this study include Anti-SGK196 (ab57908, Abcam, Cambridge, UK); Anti-GSK3β (A0480, Abclonal, Wuhan, China), Anti-p-GSK3β-S9 (AP0039, Abclonal); Anti-RPN1 (sc48367, Santa Cruz, Dallas, TX); Anti-p-AKT-Ser473 (#4058, Cell Signaling Technology, Danvers, MA), Anti-p-AKT-Thr308 (#13038, Cell Signaling Technology), Anti-AKT(pan) (#2920, Cell Signaling Technology), Anti-Snail (#3879, Cell Signaling Technology); Anti-HA (H6908, Sigma-ALDRICH, MO), Anti-Flag (F1804, Sigma-ALDRICH), Vimentin (sc-66001,Santa Cruz), α-dystroglycan (VIA4) (sc-53986, Santa Cruz) and Anti-GAPDH (HC301-01, TRANSGEN BIOTECH, Beijing, Shanghai). All antibodies for Western blotting were used at dilution 1:1000. For Immunofluorescence assays, Anti-HA (H6908, Sigma-ALDRICH) was used at dilution 1:100. Other reagents include DMEM medium (11965-084, Gibco), RPMI 1640 medium (11875-085, Gibco), Cell Counting Kit reagents (40203ES60, YEASEN), puromycin (P8230, Solarbio, Beijing, China), LY294002 (L9908, Sigma-ALDRICH), CHX (5087390001, Sigma-ALDRICH), Protease inhibitor Cocktail (EDTA-Free, 100x in DMSO, 20124ES03, YEASEN), Endo H (# P0702S, New England Biolabs, Ipswich, MA), and PNGase F (# P0705S, New England Biolabs).
Xu C., Zhang M., Bian L., Li Y., Yao Y, & Li D. (2020). N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells. Oncogenesis, 9(1), 4.
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2

Cellular Stress and Autophagy Modulation

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6-hydroxydopamine hydrochloride (6-OHDA; H4381), catalase (C8531), rotenone (R8875), MRT68921 (SML1644), and trehalose (T9531) were obtained from Sigma-Aldrich. Bafilomycin A1 (023–11641), benzoquinone (171–00242), H2O2 (080–01186), N-acetyl-L-cysteine (NAC; 017–05131), and Z-VAD(OMe)-FMK (zVAD; 269–02071) were from Wako. Brefeldin A (203729) was from Merck. Ac-YVAD-CMK (YVAD; 10014) was from Cayman Chemical Company. Rapamycin (S1039) was obtained from Selleck Chemicals. The following antibodies were from commercial sources: anti-PARK7/DJ-1 (ab4150), anti-CTSB (abcam, ab58802); anti-RPN1 (Santa Cruz Biotechnology, sc-12164); anti-FN1 (610077), anti-CAV1 (BD Biosciences, 610060); anti-VDAC1 (Merck, AP1059); anti-LMNA (2032), anti-CASP3 (9662), anti-ATG16L1 (8089), anti-phospho-AMPK Thr172 (2535), anti-phospho-ULK1 Ser555 (5869), anti-phospho-RPS6KB1/p70S6K Thr389 (Cell Signaling Technology, 9234); and anti-LC3B (L7543), and anti-ACTB (Sigma-Aldrich, A5441). Monoclonal antibody against PARK7 (Clone 3843) was prepared as previously described [62]. All other chemicals, of analytical grade, were obtained from Sigma-Aldrich or Wako.
Urano Y., Mori C., Fuji A., Konno K., Yamamoto T., Yashirogi S., Ando M., Saito Y, & Noguchi N. (2018). 6-Hydroxydopamine induces secretion of PARK7/DJ-1 via autophagy-based unconventional secretory pathway. Autophagy, 14(11), 1943-1958.
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