Shhgfp cre

All animal experiments were carried out according to protocols approved by the Institutional Animal Care and Use Committee at Harvard Medical School. Mouse lines are described in published literatures and purchased from the Jackson Laboratory: ShhGFP-Cre (Jackson Laboratory stock no. 005622), Prrx1Cre (Jackson Laboratory stock no. 005584), Sox2CreER (Jackson Laboratory stock no. 017593), tdTMT^tg/+(Rosa26-TdTomato; Jackson Laboratory stock no. 007909), Taz^f/f/Yap^f/f (Jackson Laboratory stock no. 030532), conditional Yap^* or Yap^gof (Rosa26^{lox-stop-lox-rtTA/+};
Col1a1^{Teto-YapS127A/+}) (38 (link)), Ptch1^LacZ (53 (link)), and Ctgf-GFP (38 (link)). Timed mating of heterozygous intercrosses was performed to generate embryos of the indicated embryonic stage. The day in which a vaginal plug was confirmed was designated as E0.5. Mutant and transgenic embryos were processed in parallel with littermate controls. For the ShhCre;Yap^* embryos, Dox was administered to pregnant female mice at 0.2 mg/ml in drinking water starting at E8.5. For Sox2CreER;Yap^* embryos, Dox (same as above) and TM were administered at E7.5 (via intraperitoneal injection of the pregnant females (75 mg/kg of body weight).

Shhgfpcre (JAX stock #05622), Piezo1flox (JAX stock #029213), Piezo2flox (JAX stock #027720) and Rosa26-CAG-loxp-stop-loxp-tdTomato (JAX stock #007914) were obtained from Jackson Laboratory. Data reported herein have been compiled from the examination of multiple embryonic and postnatal time points for wildtype (WT), heterozygous and conditional knockout animals. Both male and female mice were used in this study; experimental comparisons that underwent naphthalene injury controlled for sex. Pregnant dams and adults were killed for experiments through CO2 asphyxiation and cervical dislocation followed by isolation of vital postnatal organs and embryos. Fetal and neonate mice (< 10 days) were put on ice until motionless and euthanized via decapitation. Timed pregnancies were confirmed through visualization of vaginal plugs with noon on the day plugs were detected designated as E0.5. Mutant and control animals were studied at E15.5, E18.5, P0, P15 and 6 weeks of age. Mice were bred and housed in the University of Wisconsin-Madison Biomedical Research Model Services Laboratory. Animal procedures were approved by the University of Wisconsin-Madison Institutional Animal Care and Use Committee and conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals [55 ].