Gsdmd antibody

Manufactured by Affinity Biosciences

Sourced in United States, China

The GSDMD antibody is a laboratory reagent used for the detection and analysis of the GSDMD (Gasdermin D) protein. GSDMD is a key mediator of pyroptosis, a type of programmed cell death. The antibody can be used in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of GSDMD in biological samples.

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Lab products found in correlation

Anti il 1β antibody, by Cell Signaling Technology (1 mentions) Ml264, by MedChemExpress (1 mentions) Caspase 1 antibody, by Cell Signaling Technology (1 mentions) Acss2 inhibitor, by Selleck Chemicals (1 mentions) F4 80 antibody, by Proteintech (1 mentions) Disulfiram, by Merck Group (1 mentions) Bicinchoninic acid bca protein assay kit, by Meilun (1 mentions) Anti β actin antibody, by Beyotime (1 mentions) Hif 1α ab1, by Abcam (1 mentions) Total glutathione peroxidase gpx assay kit with nadph, by Beyotime (1 mentions) Collagenase 4, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Ripa lysis buffer, by Meilun (1 mentions) Malondialdehyde mda assay kit, by Beyotime (1 mentions)

Close spelling variants of this product

GSDMD (11 citations)

2 protocols using gsdmd antibody

LPS-Induced Inflammation Mechanism

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Lipopolysaccharides (Escherichia coli O111:B4, L2630, for animal experiments; Escherichia coli O111:B4, L4391, for cell experiments) were purchased from Merck (New Jersey, USA). ACSS2 inhibitor (cat no: S8588) was purchased from Selleck (Houston, USA). ML264 (cat no: HY-19994) was purchased from MedChemExpress (New Jersey, USA). Lotus Tetragonolobus Lectin (LTL) (cat no: FL-1321-2) was purchased from Thermo Fisher (MA, USA).
The following antibodies were used. ACSS2 antibody (cat no: ab133664) from Abcam (Cambridge, UK); KIM-1 antibody (cat no: sc-518008) from Santa Cruz (California, USA); F4/80 antibody (cat no: 28463-1-AP) from Proteintech (Wuhan, China); Cleaved GSDMD (N Terminal) antibody (cat no: A22523) and NLRP3 antibody (cat no: A5652) from Abclonal (Wuhan, China); caspase-1 antibody (cat no: 24232), anti-IL-1β antibody (cat no: 12242) from Cell Signaling Technology (Massachusetts, USA); GSDMD antibody (cat no: AF4012), NF-κB p65 antibody (cat no: AF5006), phospho-NF-κB p65 (Ser536) antibody (cat no: AF2006), and KLF5 antibody (cat no: AF7542) from Affinity (Changzhou, China).

Lu J., Hou Y., Liu S.X., Jin B., Liu J., Li N., Zhu Y., Zhang Q.Y., Wan C., Feng Y., Xie J, & Jiang C.M. (2024). Acetyl-CoA synthetase 2 induces pyroptosis and inflammation of renal epithelial tubular cells in sepsis-induced acute kidney injury by upregulating the KLF5/NF-κB pathway. Cell Communication and Signaling : CCS, 22, 187.

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Comprehensive Protein Analysis Protocol

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Bicinchoninic acid (BCA) protein assay kit (#MA0082-1) and RIPA lysis buffer (#MA0153) were purchased from Meilunbio (Dalian, China). L-arginine (L-Arg) monohydrochloride (#A6969), Collagenase IV (#C9407), Disulfiram (#HY-B0240) and DAPI (#D9542) were from Sigma-Aldrich (St. Louis, MO, USA) of Merck. GSDMD antibody (#AF4012) was from Affinity (Shanghai, China), REDD1 (Regulated in Development and DNA Damage Responses 1). Antibody (#sc-376671) and p-NF-Kb (p-P65) (#sc-398442) were from Santa Cruz Biotechnology (CA, USA). Resveratrol (#A2122398) was from Aladdin (Shanghai, China). TXNIP(#ab188865) and HIF-1α (#ab1) were from Abcam (Boston, MA, USA). Malondialdehyde (MDA) assay kit (#S0131S), catalase (CAT) (#S0051) assay kit, total glutathione peroxidase (GPX) assay kit with NADPH (#S0058) and anti-β-actin antibody (AF0003) were purchased from Beyotime Biotech (Shanghai, China). Mouse IL-1β ELISA kit (E-EL-M0037c) was purchased from Elabscience Biotechnology Co. Ltd. (Wuhan, China). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) of Merck.

Zhang C., Niu H., Wan C., Yu X., Xin G., Zhu Y., Wei Z., Li F., Wang Y., Zhang K., Li S., Dong Y., Li Y, & Huang W. (2022). Drug D, a Diosgenin Derive, Inhibits L-Arginine-Induced Acute Pancreatitis through Meditating GSDMD in the Endoplasmic Reticulum via the TXNIP/HIF-1α Pathway. Nutrients, 14(13), 2591.

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