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Antibodies against caspase 9

Manufactured by Cell Signaling Technology
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Antibodies against caspase-9 are laboratory reagents used to detect and study the expression and activity of caspase-9, a key enzyme involved in the apoptosis (programmed cell death) signaling pathway. These antibodies can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to help researchers investigate cellular processes related to apoptosis.

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Lab products found in correlation

Phospho akt, by Cell Signaling Technology (2 mentions) Caspase 3, by Cell Signaling Technology (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Annexin 5 fitc, by BD (1 mentions) Jc 1 mitoscreen kit, by BD (1 mentions) Scrambled control sirna, by Qiagen (1 mentions) Gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Caspase 8, by Cell Signaling Technology (1 mentions) Embelin, by Bio-Techne (1 mentions) Propidium iodide staining solution, by BD (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Hoechst 33342 solution, by BD (1 mentions) Cytofix cytoperm plus fixation and permeabilization solution kit, by BD (1 mentions) Xiap antibody, by Abcam (1 mentions) Cytochrome c, by Santa Cruz Biotechnology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Cleaved caspase 9, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Antibody against ki67, by Abcam (1 mentions)

Close spelling variants of this product

Caspase-9 (660 citations) Antibodies against caspase-3 and caspase-9 (3 citations) Antibodies against cleaved caspase-9 (3 citations) Caspases (2 citations) Primary antibodies against Caspase-9 (2 citations) Primary antibodies against caspase-3 and caspase-9 (2 citations)

3 protocols using antibodies against caspase 9

1

Embelin-induced Apoptosis Pathway Analysis

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Embelin was purchased from Tocris Bioscience (Minneapolis, MN). zVAD-fmk was purchased from Calbiochem (San Diego, CA). Antibodies against caspase-9, caspase-8, Bid, Bcl-xL, phospho AKT and cleaved caspase-3, caspase-3 were purchased from Cell Signaling Technologies (Beverly, MA). Cytochrome c, PARP and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). XIAP antibody was purchased from Abcam (Cambridge, England). Annexin V-FITC, Propidium iodide staining solution, Hoechst 33342 Solution, BD Cytofix/Cytoperm plus fixation and permeabilization solution kit, PE Active Caspase-3 Apoptosis Kit and Apoptosis, BD MitoScreen (JC-1) Kit, DNA Damage and Cell Proliferation Kit were purchased from BD Biosciences (NJ, USA). AKT, XIAP and Scrambled control siRNA were purchased from Qiagen (Valencia, CA, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA). 5-(2-Benzothiazolyl)-3-ethyl-2-[2-(methylphenylamino)ethenyl]-1-phenyl-1H-benzimidazolium iodide was purchased from Sigma Aldrich (USA).
Prabhu K.S., Siveen K.S., Kuttikrishnan S., Iskandarani A., Tsakou M., Achkar I.W., Therachiyil L., Krishnankutty R., Parray A., Kulinski M., Merhi M., Dermime S., Mohammad R.M, & Uddin S. (2017). Targeting of X-linked inhibitor of apoptosis protein and PI3-kinase/AKT signaling by embelin suppresses growth of leukemic cells. PLoS ONE, 12(7), e0180895.
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2

Antibody Immunoblot Analysis Protocol

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Whereas antibodies against caspase-9, cleaved caspase-9, caspase-3, cleaved caspase-3, PARP, cleaved PARP, H3, β-actin were purchased from Cell Signaling Technology, antibodies against AR, EZH2 and H3K27me3 were obtained from Active Motif. Antibody against Ki-67 was purchased from Abcam.
Kong Y., Zhang Y., Mao F., Zhang Z., Li Z., Wang R., Liu J, & Liu X. (2020). Inhibition of EZH2 enhances the antitumor efficacy of metformin in prostate cancer. Molecular cancer therapeutics, 19(12), 2490-2501.
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3

Curcuma zedoaria Anticancer Activity

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The rhizome powder of Curcuma zedoaria (Christm.) Roscoe was obtained from Uchida Wakanyaku Ltd. (Tokyo, Japan). TE-8 cells were purchased from RIkEN BioResource Center (Ibaraki, Japan) and HET-1A cells were from American Type Culture Collection (Manassas, VA, USA). Balb/c nude mice (6-week-old, female) were purchased from Nihon CLEA (Tokyo, Japan). Antibodies against caspase-9, caspase-3, PARP, Bcl-2, PTEN, phospho-Akt, phospho-mTOR, phospho-STAT3, FGFR1 and MMP-2 were obtained from Cell Signaling Technology Inc. (danvers, MA, USA). Anti-β-actin and dMSO were purchased from Sigma (St. Louis, MO, USA).
Hadisaputri Y.E., Miyazaki T., Suzuki S., Kubo N., Zuhrotun A., Yokobori T., Abdulah R., Yazawa S, & Kuwano H. (2015). Molecular characterization of antitumor effects of the rhizome extract from Curcuma zedoaria on human esophageal carcinoma cells. International journal of oncology, 47(6).
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