Synr premix ex taqtm kit

A 0.1-g sample of wheat (Triticum aestivum L.) leaves was used to extract total RNA by using a Total RNA kit (TIAGEN, Beijing, China) following the manufacturer’s instructions. For the complementary DNA (cDNA) synthesis, 2 mg of total RNA was reverse-transcribed using a PrimeScript TM II First-Strand cDNA synthesis Kit (TakaRa Dalian, China). The quantitative real-time PCR (qRT-PCR) analysis was performed with a Light Cycle 480 II system (Roche, Basel, Switzerland) using an SYNR Premix Ex TaqTM kit (TakaRa Dalian, China). The UBI-eq gene was used as an internal control. The selected stress-responsive genes were from wheat (Triticum aestivum L.). The primer sequences used for the PCR are given in Table S1 (Supplementary Materials). Three replicates were made for three separate RNA extracts from the three samples.

Total RNA was isolated by using a total RNA kit (TIAGEN, Beijing, China) following the manufacturer’s instructions, followed by [88 (link),89 (link)]. For the complementary DNA (cDNA) synthesis, 2 mg of total RNA was reverse-transcribed using a Prime-Script TM II First-Strand cDNA synthesis Kit (TakaRa, Dalian, China). The quantitative real-time PCR (qRT-PCR) analysis was performed with a Light Cycle 480 II system (Roche, Basel, Switzerland) using an SYNR Premix Ex TaqTM kit (TakaRa, Dalian, China). The UBI-eq gene was used as an internal control. The primer sequences used for the PCR are given in (Supplementary File S5, sheet 2). Three replicates were made for three separate RNA extracts from the three samples.