Propac wcx 10g biolc guard column

The chemical stability of the mAb was analyzed using a Thermo Scientific™ Dionex™ UltiMate™ 3000 UHPLC system equipped with a VWD-3400RS UV/Vis absorbance detector and a ProPac™ WCX-10G BioLC™ analytical column (4 × 250 mm) equipped with a ProPac™ WCX-10G BioLC™ guard column (4 × 50 mm), all from Thermo Fisher Scientific (Waltham, MA, USA). Mobile phase A consisted of 20 mM TRIS (pH 8.0), whereas mobile phase B contained 20 mM of TRIS and 300 mM of sodium chloride (pH 8.0). Elution was performed in a linear salt gradient mode from 0% B to 20% B in 30 min with a flow rate of 1 mL/min. Before analysis, the samples were diluted to a mAb concentration of 0.1 g/L with mobile phase A, and the injection volume was 100 µL. Elution was detected at 280 nm, and the integration of the chromatograms was performed with Chromeleon™ 7.2.7 (Thermo Fisher Scientific, Waltham, MA, USA). The peak areas were divided into three components: the main peak, acidic variants corresponding to every peak eluting before the main peak, and basic variants corresponding to every peak eluting after the main peak.

A Thermo Scientific™ Dionex™ UltiMate™ 3000 UHPLC system, featuring a VWD-3400RS UV/Vis absorbance detector and equipped with a ProPac™ WCX-10G BioLC™ analytical column (4 × 250 mm) together with a ProPac™ WCX-10G BioLC™ guard column (4 × 50 mm), all from Thermo Fisher Scientific (Waltham, MA, USA), was utilized to examine the chemical stability of LMU1. Mobile phase A was composed of 20 mM TRIS (pH 8.0), while mobile phase B consisted of 20 mM TRIS and 300 mM sodium chloride (pH 8.0). A linear salt gradient mode was used for elution, ranging from 0% B to 20% B over 30 min at a flow rate of 1 mL/min. Prior to analysis, samples were diluted 1:100 using mobile phase A, and the injection volume was 10 µL or 100 µL depending on the mAb concentration. Detection of elution occurred at 280 nm, and chromatogram integration was carried out using Chromeleon™ 7.2.7 software (Thermo Fisher Scientific, Waltham, MA, USA). The integrated chromatograms were categorized into three components: the main peak, acidic variants associated with each peak that eluted prior to the main peak, and basic variants linked to each peak that eluted after the main peak.