The concentration of cytokines in the serum or culture supernatants was measured by ELISA (enzyme-linked immunosorbent assay) using the kit for TNF-α (Mybiosource # MBS9135747, San Diego, CA, USA), IFN-γ (Mybiosource # MBS9135739), GM-CSF (Mybiosource # MBS9135768), IL-6 (Mybiosource # MBS9135728), and IL-10 (Mybiosource # MBS9135736).
Gm csf
Manufactured by MyBioSource
Sourced in United States
GM-CSF, also known as granulocyte-macrophage colony-stimulating factor, is a protein that plays a crucial role in the regulation and differentiation of various immune cells, including granulocytes and macrophages. It is a key factor in the production and function of these cells, which are essential for the body's immune response.
Automatically generated - may contain errors
2 protocols using gm csf
1
Cytokine Profiling by ELISA
2
Generation of Murine Dendritic Cells
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Bone marrow harvested from the leg bones of naïve C57BL/6 mice was cultured for 7 days in complete RPMI and 20 ng/mL GM-CSF (MyBiosource, San Diego, CA, USA) as described [15 (link)]. For OVA vaccinations, cells were pulsed with OVA protein overnight on day 5 at a final concentration of 200 μg/mL followed by 1 μg/mL of lipopolysaccharide (Sigma-Aldrich) overnight on day 6. Dendritic cells were MHCII+ and CD80hi.
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