The distillated organic solvents of n-hexane, ethyl acetic, and methanol were used for sample extraction. For the antioxidant assay, SOD reagents nitro blue tetrazolium (NBT), riboflavin, N,N,N’,N’-tetramethyl ethylenediamine (TEMED), and PBS were purchased from Amresco. The thin-layer chromatography (TLC) spray reagent, 10% sulfuric acid in ethanol, used in this study was purchased from Merck and Sigma-Aldrich. Kiesel G 60 silica gel resins and ODS columns of LiChroprep RP-18 (Merck, Darmstadt, Germany) were used for column chromatography. TLC analysis was performed using Kiesel gel 60 F254 and RP-18 F254S (Merck). Deuterated solvents were purchased from Merck Co., Ltd., and Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
Kiesel g 60 silica gel resins
Manufactured by Merck Group
Sourced in Germany
Kiesel G 60 silica gel resins are a type of chromatographic packing material used for liquid chromatography. They are composed of silica gel with a particle size of 60 micrometers. The core function of Kiesel G 60 silica gel resins is to provide a stationary phase for the separation and purification of various chemical compounds.
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2 protocols using kiesel g 60 silica gel resins
1
Antioxidant Extraction and Analysis
2
Buah Merah Purification Protocol
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Fresh Buah Merah (P. Conodieus Lam.) was collected from Papua, Indonesia, in June 2017. The sample was identified at the Laboratory of Plants Taxonomy -Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Bandung, Indonesia. Materials for the purification of the compounds used Kiesel G 60 silica gel resins (No. Cat 1.07734.1000, Merck) and the ODS of LiChroprep RP-18 (Merck, Darmstadt, Germany). Meanwhile, the Kiesel gel 60 F 254 and RP-18 F 254S (Merck) are used for Thin-Layer Chromatography analysis. Then, deuterated solvents were purchased from Merck Co. Ltd. and Sigma Aldrich Co. Ltd. (St. Louis, MO, USA).
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