Antibodies were from the following sources: CD4 Alexa 488 or Alexa 405 (GK1.5), TS1 Alexa 647 (6.5) and F4/80 Alexa 488 (HB-198) were lab-prepared. CD25 (R-phycoerythrin (PE) (7D4)) was from Southern Biotech. FOXP3 (150D), CD62L PE-Cy7 (Mel-14), PD-1 PE (29F.1A12), CD8± PE (53-6.7) and CD44 APC-Cy7 (1M7) were from BioLegend. Gr-1 PE (RB6-8C5) and CD11b PE (MI/70) were from eBioscience. CD4 PE (RM4-5; BD) and CD8 PE (YTSI56.7.7; Biolegend), which could bind in the presence of the depleting antibodies, were used for confirmation of in vivo T cell depletions. Ethidium monoazide (EMA, Invitrogen) or propidium iodide were used to exclude dead cells.
Cd4 pe rm4 5
Manufactured by BD
CD4 PE (RM4-5) is a fluorochrome-conjugated antibody used in flow cytometry applications to identify and quantify CD4-positive cells within a sample. The product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Ethidium monoazide ema, by Thermo Fisher Scientific (1 mentions)
Cd8 pe 53 6.7, by BioLegend (1 mentions)
Efluor 780, by Thermo Fisher Scientific (1 mentions)
Zombie aqua, by BioLegend (1 mentions)
Cd25 pe, by Thermo Fisher Scientific (1 mentions)
Cd8a fitc, by Thermo Fisher Scientific (1 mentions)
Cd8a apc cy7, by BioLegend (1 mentions)
Cd8a pe cy7, by BioLegend (1 mentions)
Cd8a pe, by BD (1 mentions)
Cd19 apc cy7, by BioLegend (1 mentions)
Cd127 apc, by BioLegend (1 mentions)
Cd45.1 percp cy5 5 a20, by BioLegend (1 mentions)
Tnfα pe mp6 xt22, by BD (1 mentions)
Ifn γ apc xmg1, by BioLegend (1 mentions)
2 protocols using cd4 pe rm4 5
1
Multiparametric Analysis of Immune Cell Subsets
2
Multiparametric Phenotyping of CD8+ T Cells
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Gp33‐41 peptide (KAVYNFATM) and gp61‐80 (GLNGPDIYKGVYQFKSVEFD) peptides were purchased from Mimotopes. PE‐conjugated gp33‐MHC class I tetramer (H‐2Db/gp33‐41) was kindly provided by the NIH tetramer core facility. For exclusion of dead cells, efluor780 (eBioscience) or ZombieAqua (BioLegend) was used. Antibodies used were as follows: PD‐1‐FITC (J43; Thermo Fisher Scientific), HVEM‐APC (LH1; eBioscience), CD19‐APC‐Cy7 (6D5; BioLegend), CD4‐PE (RM4‐5; BD Bioscience), CD4‐PerCP‐Cy5.5 (RM4‐5; BioLegend), CD8a‐FITC (53‐6.7, eBioscience), CD8a‐PE‐Cy7 (53‐6.7; BioLegend), CD8a‐APC‐Cy7 (53‐6.7; BioLegend), CD8a‐PE (53‐6.7; BD Bioscience), IFNγ‐APC (XMG1.2; BioLegend), CD127‐APC (SB/199; BioLegend), CD25‐PE (PC61; eBioscience), Ki‐67‐PE (16A8; BioLegend), KLRG‐1‐PerCP‐eFluor710 (2F1; eBioscience), CD62L‐BV421 (MEL‐14; BioLegend), CD45.1‐PerCP‐Cy5.5 (A20; BioLegend), TNFα‐PE (MP6‐XT22; BD Bioscience), and TNFα‐FITC (MP6‐XT22; BioLegend).
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