The UHMWPE sample chips were irradiated using a Mark I 68A Cs-137 Irradiator, manufactured by JL Shepherd and Associates (San Fernando, CA). X-ray photoelectron spectroscopy data was acquired with a Physical Electronics (PHI) XPS 5000 Versaprobe. Samples were sputter-coated with 1-2 nm of gold before scanning electron microscope (SEM) images were acquired on a Zeiss Merlin Scanning Electron Microscope operating at 10 keV and 8 mm working distance. Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) was performed using a Nicolet™ iS™ 5 FT-IR Spectrometer with an iD5 ATR Accessory equipped with OMNIC™ FTIR Software (Thermo Scientific, USA). Samples were pressed against the diamond tip, and resulting spectra represent the average over 32 scans. Epi-fluorescent images of Cy5 dye coatings were collected on a Nikon AZ100 Upright Wide Field Microscope equipped with NIS-Elements Software with a 5× objective and Cy5 filter at 3.0× zoom (0.72 μm/pixel).
Omnic ftir software
Manufactured by Thermo Fisher Scientific
Sourced in United States
OMNIC™ FTIR Software is a powerful analytical software designed for Fourier Transform Infrared (FTIR) spectroscopy. It provides a comprehensive suite of tools for data acquisition, processing, and analysis of FTIR spectra.
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2 protocols using omnic ftir software
1
UHMWPE Characterization by Multimodal Analysis
2
FTIR Analysis of Pine Wood Samples
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Fourier transform infrared spectroscopy was carried out using an attenuated total reflection mode on a Thermo Fischer FTIR spectrometer (Nicolet iS50), with range 4000–400 cm−1. The samples used for this part of the study included those from sound pine, an isopropanol-treated control, the treatment groups, as well as pure polymer samples. A resolution of 4 cm−1 was used and each spectrum was derived from 32 scans. Three spectra from each sampling site were taken and averaged. The wood samples were analysed at four points: the surface, the core and between the surface and core both along and across the grain (Fig. S2 ). Thermo Scientific OMNIC FTIR software was used to analyse the data. Spectra were baseline corrected and normalised to 1508 cm−1 (wood spectra) or 1725 cm−1 (pure polymer spectra).
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