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Sigenome smartpool sirna reagent

Manufactured by Horizon Discovery
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SiGENOME SMARTpool siRNA reagents are a collection of pre-designed, pooled small interfering RNA (siRNA) molecules. These reagents are designed to target and silence specific genes, enabling researchers to study gene function and expression.

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Lab products found in correlation

Trypsin edta, by Thermo Fisher Scientific (3 mentions) Sicontrol nontargeting sirna, by Horizon Discovery (2 mentions) Dharmafect 1 transfection reagent, by Horizon Discovery (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Sigenome non targeting sirna pool 2, by Horizon Discovery (1 mentions) Bez235, by Chemietek (1 mentions) Dharmafect sirna transfection reagent, by Horizon Discovery (1 mentions) Non targeting sirna, by Horizon Discovery (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Opti mem reduced serum media, by Thermo Fisher Scientific (1 mentions)

6 protocols using sigenome smartpool sirna reagent

1

DAPK2 Knockdown Protocol

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DAPK2 expression was transiently knocked down in cells using siGENOME SMARTpool siRNA reagent (Dharmacon, Lafayette, CO, USA) against human DAPK2 (NM_014326). For control siRNA, siCONTROL non-targeting siRNA was used (Dharmacon).
Shiloh R., Gilad Y., Ber Y., Eisenstein M., Aweida D., Bialik S., Cohen S, & Kimchi A. (2018). Non-canonical activation of DAPK2 by AMPK constitutes a new pathway linking metabolic stress to autophagy. Nature Communications, 9, 1759.
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2

Transient DAPK2 Knockdown Using siRNA

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DAPK2 expression was transiently knocked down in cells using siGENOME SMARTpool siRNA reagent (Dharmacon, Lafayette, CO, USA) against human DAPK2 (NM_014326). For control siRNA, siCONTROL non-targeting siRNA was used (Dharmacon). shRNA targeting DAPK2 (nucleotides 563–581, NM_014326) and HcRed (nucleotides 99–117, AF363776) were designed in our lab. The 19-mer oligos were annealed and ligated into the BglII and HindIII of pSUPER-based shRNA vector.
Ber Y., Shiloh R., Gilad Y., Degani N., Bialik S, & Kimchi A. (2014). DAPK2 is a novel regulator of mTORC1 activity and autophagy. Cell Death and Differentiation, 22(3), 465-475.
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3

siRNA-Mediated Knockdown of Oncogenes in Ovarian Cancer

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We used siGENOME SMARTpool siRNA reagents (Dharmacon) for the RNAi-mediated knockdown of human EGFR, ERBB2, ERBB4, and ABCB1. Ovarian cancer cell lines were transiently transfected with 15 nM siRNA, including the siGENOME Non-Targeting siRNA Pool #2, using DharmaFECT1 transfection reagent (Dharmacon) according to the manufacturer’s instructions. Cells were harvested 24 hours after transfection and either seeded in 96-well microplates for cell proliferation assays or re-plated for expansion prior to RNA extraction.
McCorkle J.R., Gorski J.W., Liu J., Riggs M.B., McDowell A.B., Lin N., Wang C., Ueland F.R, & Kolesar J.M. (2021). Lapatinib and poziotinib overcome ABCB1-mediated paclitaxel resistance in ovarian cancer. PLoS ONE, 16(8), e0254205.
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4

Differential PI3K/Akt Pathway Modulation

Cited 1 time
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Growth factors EGF, PDGF, and IGF-1 were obtained from Calbiochem (Merck (Schweiz) AG, Zug, Switzerland). YM024 [30] (link), PIK75 [31] (link), TGX221 [32] (link), and IC87114 [33] (link) were generously provided by Professor S. P. Jackson (The Australian Centre for Blood Diseases, Monash University, Melbourne, Australia). A66 was previously described [34] (link). BEZ235 [35] (link) was obtained from ChemieTek (Indianapolis, IN, USA). For growth factor stimulation, cells were pretreated with 0, 1 μM YM024, or 1 μM TGX221 for 1 h. GBM cells were transfected with siRNA pools (siGENOME SMART pool siRNA reagents; Dharmacon, Lafayette, CO, USA) targeting PI3K p110α, PI3K p110β, PI3K p110δ, Akt1, Akt2, Akt3, S6K1, or Rac1 using DharmaFECT siRNA transfection reagents (Dharmacon, Lafayette, CO, USA) according to the manufacturer’s protocol. Dharmacon’s siGENOME Non-Targeting siRNA Pool #2 (SCR) and TOX transfection control (TOX) were used as negative, non-targeting and positive transfection control, respectively.
Höland K., Boller D., Hagel C., Dolski S., Treszl A., Pardo O.E., Ćwiek P., Salm F., Leni Z., Shepherd P.R., Styp-Rekowska B., Djonov V., von Bueren A.O., Frei K, & Arcaro A. (2014). Targeting Class IA PI3K Isoforms Selectively Impairs Cell Growth, Survival, and Migration in Glioblastoma. PLoS ONE, 9(4), e94132.
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5

siRNA Knockdown of Tet and Sim2

Cited 2 times
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siRNA knockdown was carried out as previously described (24 (link), 32 (link)). Briefly, siRNAi against Tet1, Tet2, Tet3, or Sim2 was performed using siGenome SMART pool siRNA reagents (Dharmacon). Nontargeting siRNA (Dharmacon) was used as control.
Song C., Wang L., Wu X., Wang K., Xie D., Xiao Q., Li S., Jiang K., Liao L., Yates JR I.I.I., Lee J.D, & Yang Q. (2018). PML recruits TET2 to regulate DNA modification and cell proliferation in response to chemotherapeutic agent. Cancer research, 78(10), 2475-2489.
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6

RNA interference for gene knockdown

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Reagents are as follows: siGENOME SMARTpool siRNA reagents (Dharmacon) for negative control (NC) (siNC), PABPC1L (siPABPC1L) and MYC (siMYC); DharmaFECT 1 transfection reagent (Dharmacon); Trizol reagent (ThermoFisher), Dulbecco’s modified Eagle medium (DMEM) (ThermoFisher), OptiMem reduced serum media (ThermoFisher), foetal bovine serum (FBS) (ThermoFisher), Trypsin-EDTA (ThermoFisher).
Zhang B., Babu K.R., Lim C.Y., Kwok Z.H., Li J., Zhou S., Yang H, & Tay Y. (2019). A comprehensive expression landscape of RNA-binding proteins (RBPs) across 16 human cancer types. RNA Biology, 17(2), 211-226.
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