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Huygens professional version 16

Manufactured by Scientific Volume Imaging
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Sourced in Netherlands

Huygens Professional version 16.10 is a software package for image deconvolution and analysis. It provides tools for processing and enhancing microscopy images, particularly those acquired with confocal and widefield microscopes.

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Lab products found in correlation

1.4 na plan apochromat objective, by Olympus (4 mentions)

Close spelling variants of this product

Huygens Professional (18 citations) Huygens Professional 16.10 (2 citations) Professional version 16.10 (2 citations)

4 protocols using huygens professional version 16

1

Confocal Imaging of Immobilized Worms

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Animals were immobilized on 2% agarose pads with 20 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC, Evolve EMCCD; Photometrics) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective at RT. A Z series of optical sections was acquired in 0.13-µm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging) and processed to yield maximum-intensity projections by using ImageJ version 1.53a (Wayne Rasband, National Institutes of Health).
Li L., Liu H., Krout M., Richmond J.E., Wang Y., Bai J., Weeratunga S., Collins B.M., Ventimiglia D., Yu Y., Xia J., Tang J., Liu J, & Hu Z. (2021). A novel dual Ca2+ sensor system regulates Ca2+-dependent neurotransmitter release. The Journal of Cell Biology, 220(4), e202008121.
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2

Spinning-Disk Confocal Imaging of C. elegans

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Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100 × 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, The Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51 n (Wayne Rasband, National Institutes of Health).
Liu H., Li L., Nedelcu D., Hall Q., Zhou L., Wang W., Yu Y., Kaplan J.M, & Hu Z. (2019). Heterodimerization of UNC-13/RIM regulates synaptic vesicle release probability but not priming in C. elegans. eLife, 8, e40585.
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3

Fluorescence Imaging of Immobilized Nematodes

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Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, the Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51n (Wayne Rasband, National Institutes of Health) (Schneider et al., 2012 (link)).
Liu H., Li L., Sheoran S., Yu Y., Richmond J.E., Xia J., Tang J., Liu J, & Hu Z. (2021). The M domain in UNC-13 regulates the probability of neurotransmitter release. Cell reports, 34(10), 108828.
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4

Fluorescence Imaging of Immobilized Nematodes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals were immobilized on 2% agarose pads with 30 mM levamisole. Fluorescence imaging was performed on a spinning-disk confocal system (3i Yokogawa W1 SDC) controlled by Slidebook 6.0 software. Animals were imaged with an Olympus 100× 1.4 NA Plan-Apochromat objective. Z series of optical sections were acquired at 0.11 μm steps. Images were deconvolved with Huygens Professional version 16.10 (Scientific Volume Imaging, the Netherlands) and then processed to yield maximum intensity projections using ImageJ 1.51n (Wayne Rasband, National Institutes of Health) (Schneider et al., 2012 (link)).
Liu H., Li L., Sheoran S., Yu Y., Richmond J.E., Xia J., Tang J., Liu J, & Hu Z. (2021). The M domain in UNC-13 regulates the probability of neurotransmitter release. Cell reports, 34(10), 108828.
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