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Cytometric bead array cba

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The Cytometric Bead Array (CBA) is a multiplex assay used for the simultaneous quantification of multiple analytes in a single sample. The core function of the CBA is to enable the detection and measurement of various proteins, cytokines, chemokines, and other biomolecules in complex biological samples.

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Lab products found in correlation

Attune nxt, by Thermo Fisher Scientific (1 mentions) Legendplex data analysis software, by BioLegend (1 mentions)

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Cytometric Bead Array (7 citations) 13-plex Cytometric bead arrays(CBA (2 citations)

3 protocols using cytometric bead array cba

1

Serum Immunoglobulin and Cytokine Analysis

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Serum levels of total IgE, IgG1, OVA-specific IgE (sIgE), and OVA-specific IgG1 (sIgG1) were determined using the commercial ELISA kits (Cayman Chemical Company) according to the instructions. Serum IL-5, IL-13, IL-2, IL-6, IL-10, IFN-γ, TNF-α, IL-4 level were determined by Cytometric Bead Array (CBA, BioLegend, Inc.).
Ma R.X., Hu J.Q., Fu W., Zhong J., Cao C., Wang C.C., Qi S.Q., Zhang X.L., Liu G.H, & Gao Y.D. (2023). Intermittent fasting protects against food allergy in a murine model via regulating gut microbiota. Frontiers in Immunology, 14, 1167562.
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2

Cytokine Release by CAR-T Cells

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To assess cytokine release by CAR-T cells, supernatants were harvested 12 hours after incubation with different types of tumor cells for assessment with a Cytometric Bead Array (CBA, Biolegend, Cat#: B314528) kit following the manufacturer’s protocol. Beads were analyzed by a flow cytometry assay. Each experiment was done in triplicate.
Li X., Feng Y., Shang F., Yu Z., Wang T., Zhang J., Song Z., Wang P., Shi B, & Wang J. (2021). Characterization of the Therapeutic Effects of Novel Chimeric Antigen Receptor T Cells Targeting CD38 on Multiple Myeloma. Frontiers in Oncology, 11, 703087.
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3

Cytokine Secretion Quantification Protocol

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In vitro cytokine secretion was measured on cell supernatants from cytotoxicity assays using BioLegend Cytometric Bead Array (CBA) flow cytometric assay and following the manufacturer’s instructions. Samples were counted on a flow cytometer (Attune NxT, ThermoFisher Scientific) and analyzed using BioLegend’s cloud-based LegendPLEX Data Analysis Software and GraphPad Prism (Version 9.3.1).
Snell D., Gunde T., Warmuth S., Chatterjee B., Brock M., Hess C., Johansson M., Simonin A., Spiga F.M., Weinert C., Kirk N., Bassler N., Campos Carrascosa L., Flückiger N., Heiz R., Wagen S., Giezendanner N., Alberti A., Yaman Y., Mahler D., Diem D., Lichtlen P, & Urech D. (2023). An engineered T-cell engager with selectivity for high mesothelin-expressing cells and activity in the presence of soluble mesothelin. Oncoimmunology, 12(1), 2233401.
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