Pooled HUVEC (from 3 to 6 individual donors, Lonza, Cologne, Germany) and HUAEC (from 250 individual donors, PeloBiotech GmbH, Planegg, Germany) were cultivated in EGM-2 Bullet Kit containing endothelial cell culture medium supplemented with 2% (v/v) fetal bovine serum, vascular endothelial growth factor, basic fibroblast growth factor, human epithelial growth factor, insulin-like growth factor-1, hydrocortisone, heparin, ascorbic acid, gentamycin, and amphotericin B (Lonza) in a standard humidified incubator at 37 °C with 5% (v/v) CO2. Cells of passage 5 were seeded in 4-well PCA chamber slides (Sarstedt, Nürnbrecht, Germany) at a cell density of 15,000 cells/well with 1 mL medium per well, and cultivated for up to 7 days. Medium was exchanged at day 2 and day 4 of cultivation.
Human epithelial growth factor
Manufactured by Lonza
Sourced in Germany
Human epithelial growth factor is a protein that stimulates the growth and differentiation of epithelial cells. It plays a critical role in the regulation of cell growth, proliferation, and survival.
Automatically generated - may contain errors
Lab products found in correlation
Hydrocortisone, by Lonza (2 mentions)
Basic fibroblast growth factor (bfgf), by Lonza (1 mentions)
Vascular endothelial growth factor (vegf), by Lonza (1 mentions)
Heparin, by Lonza (1 mentions)
Pooled huvec, by Lonza (1 mentions)
Insulin like growth factor 1, by Lonza (1 mentions)
Egm 2 bulletkit, by Lonza (1 mentions)
Gentamycin, by Lonza (1 mentions)
Amphotericin b, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Ascorbic acid, by Lonza (1 mentions)
Transferrin, by Lonza (1 mentions)
Epinephrine, by Lonza (1 mentions)
Kbm gold medium, by Lonza (1 mentions)
Insulin, by Lonza (1 mentions)
2 protocols using human epithelial growth factor
1
Endothelial Cell Culture and Characterization
2
Inflammatory Profile of Primary Keratinocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human primary adult keratinocytes were isolated from human donor surplus skin after plastic surgery. Cells from 8 different donors were used. Cells were maintained at 37°C in a humidified environment of 21% O 2 and 5% CO 2 (balance N 2 ) in keratinocyte KBM-gold medium supplemented with human epithelial growth factor, bovine pituitary extract, transferrin, epinephrine, insulin and hydrocortisone (Lonza). Cells were pre-equilibrated in supplement-free medium for 48 hours. For the characterization of the inflammatory profile, cells were treated with 50ng/ml TNF-α for 24 hours. After treatment, the medium was collected for measurement of secreted cytokines/chemokines. For the analysis of the effects of hydroxylase inhibitors on skin inflammation in vitro, cells were treated with 1mM DMOG or 100μM JNJ1935 for 1 hour and then treated with TNF-α. For HIF transcription studies the cells were transfected with the HRE-gaussia-
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