Plasma concentrations of cytokines, chemokines, and growth factors were determined using a Bio-plex Multiplex Immunoassay System (Bio-Rad, Hercules, CA, USA) using a Bio-plex ProTM Human Cytokine 27-plex assay kit as reported previously [12 (link), 13 (link)] as per the manufacturer’s instructions. Levels of 15 cytokines, including the pro-inflammatory (IL-1β, IL-5, IL-6, IL-7, IL-9, IL-15, IL-17, TNF-α), anti-inflammatory (IL1-RA, IL-4, IL-10, IL-13), and Th1 (IL-2, IFN-γ, IL-12 p70) cytokines along with seven chemokines (eotaxin, CCL-2,CCL-3,CCL-4,CCL-5, IL-8, CXCL-10) and five growth factors (basic fibroblast growth factor [FGF], G-CSF, GM-CSF, vascular endothelial growth factor [VEGF], platelet-derived growth factor-bb [PDGF-bb]), were estimated.
Bio plex protm human cytokine 27 plex assay kit
Manufactured by Bio-Rad
Sourced in United States
The Bio-plex Pro™ Human Cytokine 27-plex assay kit is a multiplex immunoassay system that enables the simultaneous quantification of 27 different human cytokines and chemokines from a single sample. The kit utilizes magnetic bead-based technology and provides a sensitive and efficient method for measuring multiple analytes in a single well.
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5 protocols using bio plex protm human cytokine 27 plex assay kit
1
Multiplex Profiling of Plasma Biomarkers
2
Cytokine Profiling in Omicron Infection
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Plasma concentrations of cytokines, chemokines, and growth factors were determined in Omicron-infected patients (n = 19) and uninfected controls (n = 36) using a Bio-plex Multiplex Immunoassay System (Bio-Rad, Hercules, CA, USA) using a Bio-plex ProTM Human Cytokine 27-plex assay kit as reported previously [24 (link)] as per the manufacturer's instructions. Levels of 15 cytokines, including the pro-inflammatory (IL-1β, IL-5, IL-6, IL-7, IL-9, IL-15, IL-17, TNF-α), anti-inflammatory (IL1-RA, IL-4, IL-10, IL-13), and Th1 (IL-2, IFN-γ, IL-12 p70) cytokines along with seven chemokines (Eotaxin, CCL-2, CCL-3, CCL-4, CCL-5, IL-8, CXCL-10) and five growth factors (basic fibroblast growth factor (FGF), G-CSF, GM-CSF, vascular endothelial growth factor (VEGF), platelet-derived growth factor-bb (PDGF-bb)) were estimated. The lowest value of the respective standards was used in the case of undetected concentrations of the cytokines, chemokines, and growth factors in the tested samples [23 (link), 24 (link)].
3
Cytokine Profiling of Patient Sera
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The patient and healthy sera were assayed using the Bio-Plex ProTM human cytokine 21-plex assay kit (Bio-Rad #MF0005KMII) and Bio-Plex ProTM human cytokine 27-plex assay kit (Bio-Rad #M500KCAF0Y) according to the manufacturer’s instructions. The raw data was processed using Bio-Plex Manager software version 6.1 (Bio-Rad Laboratories, USA). Cytokines with out-of-range (OOR) readings that were below its detection limits were recorded as 0.1 pg/ml for subsequent statistical analyses which involved log transformation of data.
4
Quantifying IL-10 Levels in Hepatitis E
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Plasma concentrations of IL-10 cytokine were determined in representative plasma samples from each category of study subjects (acute hepatitis E patients, n =20; hepatitis E recovered individuals, n =20 and healthy controls, n =27) using a Bio-plex Multiplex Immunoassay System (Bio-Rad, Hercules, CA, USA) using a Bioplex ProTM Human Cytokine 27-plex assay kit as reported previously [24 (link), 25 (link)] as per the manufacturer's instructions.
5
Cytokine Profiling of 3D and 2D HPMSCs
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Cells and tissue samples were assayed using and Bio-Plex ProTM human cytokine 27-plex assay kit (Bio-Rad#10014905) according to the manufacturer’s instructions. As for cells sample, cell supernatant from 3D- and 2D-HPMSCs at a density of 105/ml were collected after appropriate culture. As for tissue sample, 1cm-long spinal cord (cells grafts were at the center of the spinal cord) was lysed with RIPA Lysis Buffer and then quantified with Pierce bicinchoninic acid (BCA) protein assay kit (Beyotime, Shanghai, China). All the cells and tissue supernatant were next prepared for assay. Data was processed using Bio-Plex Manager software version 6.1 (Bio-Rad Laboratories, USA).
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