Juglone

AAPH (2,2′-azobis(2-methyl-propionamidine)-di- hydrochloride), BSA (bovine serum albumin), DCFH-DA(2′,7′-dichloro-dihy-drofluorescein diacetate), DPPH (2,2-diphenyl-1-picrylhydrazyl), hydrogen peroxide, MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide), TBA (thiobarbituric acid), TCA (trichloroacetic acid), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), tris-HCl, Trolox (6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid) and pyrogallol were purchased from Sigma-Aldrich (St. Louis, MO, USA). DMEM (Dulbecco’s modified Eagle’s medium), FBS (fetal bovine serum) and PBS (phosphate-buffered saline) were acquired from Gibco (Invitrogen, Paisley, UK). DNTB (5,5′-dithiobis(2-nitrobenzoic acid)) and juglone (5-hydroxy-1,4-naphthoquinone) were from Alfa Aesar (Ward Hill, MA, USA), while Folin–Cicolteau reagent was purchased from Chem-Lab (Zeldelgem, Belgium). All other general laboratory reagents were purchased from Panreac Química S.L.U. (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).

Acetonitrile (99.9%) was of an HPLC grade from Fisher Scientific (Lisbon, Portugal). Phenolic compound standards (quercetin-3-O-glucoside, myricetin, gallic acid) were from Extrasynthèse (Genay, France). Formic acid, DPPH^· (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), ATCI (acetylthiocholine iodide), acetylcholinesterase, monoamine oxidase A (MAO-A), tris-HCl and pyrogallol were purchased from Sigma-Aldrich (St. Louis, MO, USA). DNTB (5,5′-dithiobis (2-nitrobenzoic acid)), lutein, juglone (5-hydroxy-1,4-naphthoquinone), and FUDR (5-fluoro-2′-deoxyuridine) were from Alfa Aesar (Ward Hill, MA, USA). The Folin-Ciocalteu reagent was purchased from Chem-lab (Zeldelgem, Belgium). All other general laboratory reagents were purchased from Panreac Química S.L.U. (Barcelona, Spain). Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, Greenville, SC, USA).