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Ab 5500 qtrap

Manufactured by AB Sciex
Sourced in Canada

The AB-5500 QTrap is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. It is designed to provide sensitive and accurate quantitative and qualitative analysis of a wide range of analytes in complex matrices. The system combines the features of a triple quadrupole mass spectrometer with the additional functionality of a linear ion trap, allowing for advanced data acquisition modes and enhanced selectivity.

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2 protocols using ab 5500 qtrap

1

LC-MS Quantification of Paclitaxel

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Quantification of PTX was conducted on an LC-MS platform consisting of a Shimadzu High-performance liquid chromatography system and an AB-5500 QTrap (Sciex, Ontario, Canada) tandem mass spectrometer equipped with an electrospray ionization source. The sample injection volume was 5 µL, and chromatographic separation was performed on an Xbridge C18 column (3.5 µm, 50 mm × 2.1 mm internal diameter; Waters, Milford, MA, USA) at a flow rate of 400 µL/min. Mobile phase A consisted of 0.1% formic acid in water, and mobile phase B consisted of 0.1% formic acid in acetonitrile. Gradient elution started with 25% B for 0.5 minute, linearly increased to 65% at 2 minutes, to 95% B at 2.5 minute, kept at 95% B for 2 minutes, decreased to 25% B at 5 minutes, and kept at 25% for 2 minutes. The MS system was operated in positive-ion mode while a multiple reaction monitoring method was used for PTX (m/z 854.40 → 286.10) and IS (m/z 808.00 →226.00) detection. The gas temperature was 300°C with an ion-spray voltage of 5500 V, nitrogen and argon of 60 psi, declustering potential of 190.00 V for PTX and 173.00 V for IS, curtain gas of 30 psi, and collision energy of 21.00 V for PTX and 18.80 V for IS. LC-MS system control, data acquisition, peak integration, and quantitation were performed using the Analyst software package MDS SCIEX (version 1.6; Applied Biosystems, Toronto, Canada).
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2

Paclitaxel Quantification by LC-MS/MS

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Concentrations of paclitaxel were determined using an AB-5500 Qtrap (Sciex, Concord, ON, Canada) mass spectrometer with electrospray ionization source interfaced with a Shimadzu high-performance liquid chromatography system. Analyst Software (version 1.6) from Applied Biosystems (MDS SCIEX; Carlsbad, CA, U.S.A.) was used. Separation was performed on an Xbridge C18 column (50 × 2.1 mm ID, 3.5 μm; Waters, Milford, MA, U.S.A.) at a flow rate of 0.4 mL/min. The mobile phase consisted of A (100% H2O with 0.1% formic acid) and B (100% acetonitrile with 0.1% formic acid). The gradient started with 25% B for 30 s, linearly increased to 65% B at 2 min, increased to 95% B at 2.5 min, maintained at 95% B for 2 min, decreased to 25% B at 5 min, and maintained at 25% B for 2 min. The multiple reaction monitoring transitions are summarized in Table 1.
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