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The D3329 is a laboratory equipment product designed for general scientific applications. It serves as a versatile tool for researchers and technicians in various fields. The core function of the D3329 is to provide a reliable and consistent platform for conducting a range of experiments and analyses.

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Lab products found in correlation

Laminin, by Thermo Fisher Scientific (2 mentions) Goat anti mouse alexa 488, by Thermo Fisher Scientific (2 mentions) D1828, by Thermo Fisher Scientific (2 mentions) D1830, by Thermo Fisher Scientific (2 mentions) Goat serum, by Thermo Fisher Scientific (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Mouse tuj1 primary antibody, by Fortrea (2 mentions) D1844, by Thermo Fisher Scientific (1 mentions) Dmem f12 phenolredfree, by Thermo Fisher Scientific (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

3 protocols using d3329

1

Paracellular Permeability Assay

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After the TEER assay, dextran solution (10 mM HEPES [pH 7.4], 1 mM sodium pyruvate, 1 mM CaCl2, 10 mM glucose, 145 mM NaCl, 1 mM MgCl2, 0.33 mg/mL fluorescein-labeled dextran, 40 kDa [D1844; Thermo Fisher Scientific], 0.33 mg/mL Texas Red-labeled dextran, 3 kDa [D3329; Thermo Fisher Scientific]) was added to upper chamber. After 2 h of incubation at 37 °C, the solution of lower chamber was collected, and fluorescence intensity was measured using microplate reader (Ex 488 nm/Em 528 nm and Ex 590 nm/Em 615 nm).
Sakamoto H., Nishikawa M, & Yamada S. (2024). Development of tight junction-strengthening compounds using a high-throughput screening system to evaluate cell surface-localized claudin-1 in keratinocytes. Scientific Reports, 14, 3312.
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2

Fluorescent Probes and Cell Culture

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Fluo-4 (F14200), Fluo-4 AM (F14201), Dextran Texas Red, 3,000, 10,000, and 70,000 MW (D3329, D1828, and D1830, respectively) were purchased from Thermo Fisher Scientific. CT04 and CN03 were the products of Cytoskeleton Inc. Cytochalasin D (C2618), cytosine arabinofuranoside (Ara-C), poly-D-lysine, and paraformaldehyde were purchased from Millipore Sigma. NGF was purified from mouse salivary glands. Goat anti-mouse Alexa 488, 10% goat serum, and laminin (1 μg/mL) were products of Life Technologies. Mouse Tuj1 primary antibody was the product of Covance. DMEM high glucose (11,965,092), DMEM/F-12 (11,320,033), DMEM/F-12 phenol red free (21,041,025), DMEM high glucose, calcium free (21,068,028), 100X penicillin/streptomycin (10,378,016), and FBS (16,000,044) were brought from Gibco.
Yong Y., Gamage K., Cushman C., Spano A, & Deppmann C. (2020). Regulation of degenerative spheroids after injury. Scientific Reports, 10, 15472.
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3

Fluorescent Labeling of Neuronal Cells

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Fluo-4 (F14200), Fluo-4 AM (F14201), Dextran Texas Red, 3,000, 10,000, and 70,000 MW (D3329, D1828, and D1830, respectively) were purchased from Thermo Fisher Scientific. CT04 and CN03 were the products of Cytoskeleton Inc. Cytochalasin D (C2618), cytosine arabinofuranoside (Ara-C), poly-D-lysine, and paraformaldehyde were purchased from Millipore Sigma. NGF was purified from mouse salivary glands. Goat anti-mouse Alexa 488, 10% goat serum, and laminin (1 µg/mL) were products of Life Technologies. Mouse Tuj1 primary antibody was the product of Covance. DMEM high glucose (11965092), DMEM/F-12 (11320033), DMEM/F-12 phenol red free (21041025), DMEM high glucose, calcium free (21068028), 100X penicillin/streptomycin (10378016), and FBS (16000044) were brought from Gibco.
Yong Y., Gamage K.K., Cushman C., Spano A., & Deppmann C.D. (2020). Regulation of degenerative spheroids after injury.
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