Anti dykddddk beads

Manufactured by Fujifilm

Sourced in Japan

Anti-DYKDDDDK beads are a type of lab equipment used for the purification and detection of proteins that are tagged with the DYKDDDDK peptide sequence, also known as the FLAG tag. These beads are coated with an antibody that specifically binds to the FLAG tag, allowing for the efficient isolation and enrichment of the target protein from complex samples.

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Lab products found in correlation

Rabbit igg isotype control 31 235, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-DYKDDDDK (FLAG) tag antibody beads Anti-DYKDDDDK tag antibody beads Anti‐DYKDDDDK‐tag beads Anti-DYKDDDDK tag antibody magnetic beads Anti-DYKDDDDK mAb beads Anti-DYKDDDDK

2 protocols using anti dykddddk beads

Affinity Purification of FLAG-Tagged Proteins

Cited 1 time

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Soluble proteins extracted from rosette leaves of wild-type and ACR11-FLAG plants grown for 4 weeks were incubated with anti-DYKDDDDK beads (Wako, Japan) for 15 min at 25 °C. After discarding the supernatants, the beads were washed three times with ice-cold BN-solubilization buffer10 (link). Bound proteins were eluted with the BN-solubilization buffer containing DYKDDDDK peptides (Wako) or with SDS extraction buffer.

Takabayashi A., Niwata A, & Tanaka A. (2016). Direct interaction with ACR11 is necessary for post-transcriptional control of GLU1-encoded ferredoxin-dependent glutamate synthase in leaves. Scientific Reports, 6, 29668.

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Antibody Immunoprecipitation and Western Blotting

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Rabbit anti-phospho-TAZ antibody was previously described (34 (link)). Other antibodies and the reagents used in this study are as follows: mouse monoclonal anti-GFP (sc-9996), mouse monoclonal anti-poly (adp-ribose) polymerase (sc-8007), mouse monoclonal anti-CAS (H-2) (sc-271537), mouse monoclonal karyopherin α1 (187.1) (sc-101292) (Santa Cruz Biotechnology); mouse monoclonal anti-TAZ (560235) (BD Biosciences); rabbit polyclonal anti-α-tubulin (PM054) and rabbit polyclonal anti-β-actin (PM053) (Medical and Biological Laboratories Co Ltd); anti-DYKDDDDK-tag (014-22383) and anti-DYKDDDDK-beads (016-22784) (Wako Pure Chemical Industries); rabbit polyclonal anti-mCherry (GeneTex); rabbit monoclonal FoxO3a (75D8)(2497) and rabbit monoclonal YAP1(D8H1X)XP (14074) (Cell Signaling Technology); rabbit polyclonal importin α5 (18137-1-AP) and rabbit polyclonal TAZ (23306-1-AP) (Proteintech Group, Inc); mouse monoclonal β-catenin (13-8400) (Zymed Laboratory Inc); Protein G Sepharose 4 Fast Flow (17-0618-01) (GE Healthcare BioSciences); Rabbit IgG Isotype Control (31235) (Thermo Fisher Scientific); and TI-4 (2,2-dichloro-N-(4-nitrophenyl)-3-phenylcyclopropanecarboxsmide) (STK825627; Vitas-M Chemical Limited).

Nagashima S., Maruyama J., Honda K., Kondoh Y., Osada H., Nawa M., Nakahama K.I., Ishigami-Yuasa M., Kagechika H., Sugimura H., Iwasa H., Arimoto-Matsuzaki K., Nishina H, & Hata Y. (2021). CSE1L promotes nuclear accumulation of transcriptional coactivator TAZ and enhances invasiveness of human cancer cells. The Journal of Biological Chemistry, 297(1), 100803.

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