Picoprobe ldh cytotoxicity fluorometric assay kit

The LDH activity was evaluated with the PicoProbe LDH Cytotoxicity Fluorometric Assay Kit (BioVision). The LDH was measured using 10 μL of the cell supernatant to detect the LDH released from the cells after membrane damage, mixed with the buffer solution and PicoProbe up to 200 μL as in the manufacturer's protocol. The mixture was incubated in a black fluorescence 96 well plate and read at λEx/Em = 535/587 nm. The fluorescence intensity indicates the intensity of the LDH activity. The Fluorometric measurements were performed using CLARIOstar spectrophotometer (BMG Labtech). The results are expressed as mean values and the experimental groups were compared to the control group, DAMI Luc2 cells without CAR T cells in co‐culture.