Mettl14

Manufactured by ZenBio

Sourced in China

METTLER14 is a laboratory equipment product. It is a protein that plays a role in the methylation of RNA.

Automatically generated - may contain errors

Lab products found in correlation

Ripa lysis buffer, by Thermo Fisher Scientific (3 mentions) Ythdf3, by ABclonal (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Pvdf membrane, by Abcam (1 mentions) β actin, by ABclonal (1 mentions) Ythdf1, by ABclonal (1 mentions) β actin, by Abcam (1 mentions)

2 protocols using mettl14

Western Blot Analysis of m6A Regulators

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For western blot, 100 mg of fresh tissues were isolated, homogenized, and added into RIPA Lysis Buffer (Thermo Scientific, USA). After ultrasonic treatment, the proteins of the lysated samples were prepared. A total of 30 μg of protein per sample was quantified and separated by SDS-PAGE, then electrically transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, USA). The primary antibodies of METTL3 (Cat. No. 382974, Zen bioscience, China), METTL14 (Cat. No. 508530, Zen bioscience, China), FTO (Cat. No.R24362, Zen bioscience, China), YTHDF1 (Cat. No. A18126, ABclonal, China), YTHDF3 (Cat. No. A8395, ABclonal, China), and β-actin (Cat. No. ab8227, Abcam, UK) were diluted in corresponding proportion (Supplementary Table S10) and incubated with the PVDF membrane, then incubated with the secondary antibodies labeled by HRP (Cat. No. ab6721, Abcam, UK). ECL chemiluminescence method was used for testing.

Zhao G., An J., Pu Q., Geng W., Song H., Zhao Q, & Gao H. (2021). Gene Signatures and Cancer-Immune Phenotypes Based on m6A Regulators in Breast Cancer. Frontiers in Oncology, 11, 756412.

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Profiling m6A Regulators in Tissues

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For western blot, 100mg of fresh tissues were isolated, homogenized and added into RIPA Lysis Buffer (Thermo Scienti c, USA). After ultrasonic treatment, the proteins of the lysated samples were prepared.
Protein was quanti ed and separated by SDS-PAGE, then electrically transferred to a polyvinylidene uoride (PVDF) membrane (Millipore, USA). The primary antibodies of METTL3 (Zen bioscience, China), METTL14 (Zen bioscience, China), FTO (Zen bioscience, China), YTHDF1 (ABclonal, China), YTHDF3 (ABclonal, China) and β-actin (Abcam, UK) were diluted in proportion of 1:100 and incubated with the PVDF membrane, then incubated with the secondary antibodies labeled by HRP (Abcam, UK). ECL chemiluminescence method was used for testing.

Zhao G., An J., Pu Q., Geng W., Song H., Zhao Q., & Gao H. (2021). Gene Signatures And Cancer-Immune Phenotypes Based On m6A Regulators In Breast Cancer.

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