Cd71 antibody ox26

1 mL of 1% poly (ethylene glycol)-carboxylic acid (average MW 5 kDa, Sigma-Aldrich) was added to 10 mL of the GNP solution under continuous stirring at room temperature for 24 h. Then, the resulting solution was centrifuged for 30 min at 14,000 rpm to separate the unbound constituents of C-PEGylated NPs, and again the pellet was resuspended in 0.1 M phosphate-buffered saline (pH 7.4). To this solution was added 20 µL of EDC/NHS (Sigma-Aldrich) (75 mM/30 mM, v/v, 1:1) to activate the GNP solution as a coupling agent. After washing with DI water, 5 μL of the CD71 antibody (OX26) (Santa Cruz Biotechnology) was added to the resulting solution. After continuous stirring for 5 h at room temperature, the non-bonded OX26 antibodies were removed by centrifugation at 20,000 rpm for 60 min at 4°C. Finally, the pellet was resuspended in 5 mL of 0.1 M phosphate-buffered saline (pH 7.4). FTIR spectrum of dried bare and OX26@GNPs was performed by an IR Pestige-21 Shimadzu spectrometer to confirm GNP surface decoration with an OX26 antibody. However, measurement of UV–Vis spectra of the solution at 500–600 nm was carried out using a 3–5 mm quartz cuvette on a UV–Vis Perkin Elmer Lambda 25 spectrophotometer (United Kingdom).