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2 protocols using s equol

1

Synthesis and Purification of Equol Derivatives

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Daidzein (D-2946) was purchased from LC laboratories (Woburn, USA); S-(−)equol (SML2147) was purchased from Sigma-Aldrich (Germany); Equol-d4 (E593002) was purchased from Toronto Research Chemicals (TRC, Canada).
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2

Detailed Metabolic Enzyme Assay Protocol

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Daidzein, S-equol, dimethyl
sulfoxide (DMSO), glycerol, alamethicin, uridine 5′-diphosphoglucuronic
acid (UDPGA), 3′-phosphoadenosine-5′-phosphosulfate
(PAPS), and tromethamine (Tris) were obtained from Sigma-Aldrich (Zwijndrecht,
The Netherlands). DHD and O-DMA were purchased from
Cayman Chemical (AA) and Plantech (Reading), respectively. Trifluoroacetic
acid (TFA), MgCl2, 37% HCl and NaOH were obtained from
VWR (Amsterdam, The Netherlands).
Acetonitrile (ACN) and methanol
were obtained from Biosolve BV (Valkenswaard, The Netherlands). Phosphate
buffer saline (PBS) was supplied by Gibco (Paisley). Para-Pak SpinCon
concentration system was bought from Meridian Bioscience (Schijndel,
The Netherlands). Human pooled liver S9 fractions from 25 individuals
(mixed gender) were supplied by Tebu-bio (Heerhugowaard, The Netherlands).
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