Anti alcam

Manufactured by R&D Systems

Anti-ALCAM is a recombinant protein that binds to the ALCAM (Activated Leukocyte Cell Adhesion Molecule) cell surface receptor. It can be used in research applications to study ALCAM and its interactions.

Automatically generated - may contain errors

Lab products found in correlation

Cd3 fitc, by Thermo Fisher Scientific (1 mentions) Gr 1 fitc, by Thermo Fisher Scientific (1 mentions) Ter119 fitc, by Thermo Fisher Scientific (1 mentions) Nk1.1 fitc, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Anti rap1, by BD (1 mentions) Peroxidase conjugated goat anti mouse igg, by Cell Signaling Technology (1 mentions) Cxcr4 apc, by Thermo Fisher Scientific (1 mentions) Cxcr5 apc, by Thermo Fisher Scientific (1 mentions) Brilliant violet 421, by Thermo Fisher Scientific (1 mentions) Cxcl12, by R&D Systems (1 mentions) Anti sostdc1, by Abcam (1 mentions)

Spelling variants of this product

Anti-ALCAM antibody (2 citations)

2 protocols using anti alcam

Multiparametric Immune Cell Analysis

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Fluorescence-conjugated anti-mouse CD45 allophycocyanin (APC), phycoerythrin(PE) Cy7, Brilliant Violet™ 421, CD3 FITC, B220 PE, APC, Brilliant Violet™ 711, CD19 FITC, PE, APC, IgM FITC, PECy7, APC, IgD PE, CD21 FITC, CD23 PE, CD24 PECy7, CD43 PE, CD5 PECy7, CD62L FITC, GL-7 APC, CD93 APC, LFA-1 PE, VLA-4 APC, CXCR4 APC, CXCR5 APC, CD35 FITC and IL-7R APC, CD4 FITC, CD8 FITC, CD11b FITC, Gr-1 FITC, NK1.1 FITC, TER119 FITC (e-bioscience or BioLegend), anti-ALCAM (R&D Systems), anti-Rap1 (BD Biosciences), Extracellular signal-regulated kinase (ERK), p-ERK, Akt kinase (Akt), p-Akt, β-actin and peroxidase-conjugated goat anti-mouse IgG (Cell Signaling) were used for flow cytometry, immunostaining and immunoblotting. Mouse CXCL13, CXCL12 and 7α,25-OHC were purchased from R&D Systems and Sigma. NIP-APC (4-hydroxy-3-iodo-5- nitrophenylacetate-allophycocyanin) was generated in-house (34 (link)).

Ishihara S., Sato T., Sugioka R., Miwa R., Saito H., Sato R., Fukuyama H., Nakajima A., Sawai S., Kotani A, & Katagiri K. (2021). Rap1 Is Essential for B-Cell Locomotion, Germinal Center Formation and Normal B-1a Cell Population. Frontiers in Immunology, 12, 624419.

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Immunohistochemical Staining of SOSTDC1 and ALCAM

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Immunohistochemistry staining was carried out as previously described 46 . Anti-SOSTDC1
(Abcam) was used at 1:100 dilution and anti-ALCAM (R&D systems) at 1:50 dilution.

Bartolomé R.A., Pintado-Berninches L., Jaén M., de Los Ríos V., Imbaud J.I, & Casal J.I. (2020). SOSTDC1 promotes invasion and liver metastasis in colorectal cancer via interaction with ALCAM/CD166. Oncogene, 39(38).

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