Abrus seeds were purchased from B & T World Seeds (Aigues-Vives, France). Abrin was purified from Abrus precatorius seeds as described in [5 (link)]. Sequencing-grade modified trypsin was obtained from Promega Corporation (Charbonnières-les-Bains, France). RapiGest SF Surfactant was purchased from Waters Corporation (Milford, MA, USA). Dynabeads M-280 tosylactivated magnetic beads were obtained from Invitrogen (Life Technologies, Oslo, Norway). Labeled peptides for quantification were synthesized in Pepotec grade by ThermoFisher Scientific (Paisley, UK). Water (ChromaSolve LC-MS), acetonitrile (ACN, HPLC-grade) and formic acid were obtained from Honeywell/Riedel-de Haen (Seelze, Germany) and VWR chemicals (Fontenay sous Bois, France), respectively. Trifluoroacetic acid (TFA) and all other chemicals were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France) or VWR Chemicals (Fontenay sous Bois, France). For all reactions, LoBind Eppendorf tubes (Dutscher, Brumath, France) were used.
Dynabeads m 280 tosylactivated magnetic beads
Manufactured by Thermo Fisher Scientific
Sourced in Norway
Dynabeads M-280 tosylactivated are magnetic beads designed for covalent coupling of proteins, peptides, oligonucleotides, and other ligands. They have a high surface area and uniform size distribution.
Automatically generated - may contain errors
Lab products found in correlation
Sequencing grade modified trypsin, by Promega (2 mentions)
Rapigest sf surfactant, by Waters Corporation (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Formic acid, by Honeywell (1 mentions)
Deoxycholic acid, by Merck Group (1 mentions)
Disodium hydrogen phosphate, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Lc ms grade water, by Honeywell (1 mentions)
Lc ms grade acetonitrile, by Honeywell (1 mentions)
2 protocols using dynabeads m 280 tosylactivated magnetic beads
1
Purification and Characterization of Abrin
2
Quantitative Ricin Isoform Analysis
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Phosphate-buffered saline (PBS), Phosphate-buffered saline Tween 1 × (PBST 1 ×), sodium phosphate, ammonium bicarbonate, bovine serum albumin (BSA), dithiothreitol (DTT), iodoacetamide (IAA), formic acid (LC-MS grade), hydrochloric acid (HCl), sodium hydroxide, disodium hydrogen phosphate, sodium chloride, deoxycholic acid, and asialofetuin type I were from Sigma-Aldrich-Merck (Saint Quentin Fallavier, France). Water (LC-MS grade) and acetonitrile (LC-MS grade) were obtained from Honeywell/Riedel-de Haen (Seelze, Germany) and Biosolve Chimie (Dieuze, France), respectively. Sequencing-grade modified trypsin was obtained from Promega Corporation (Charbonnieres-les-Bains, France). Dynabeads M-280 tosylactivated magnetic beads were obtained from Invitrogen (Life Technologies, Oslo, Norway). Labelled peptides for quantification were synthesized in Absolute QUAntitation (AQUA) ultimate quality by ThermoFisher Scientific (Paisley, UK). For all reactions, LoBind Eppendorf tubes (Dutscher, Brumath, France) were used. Ricin isoforms D and E were obtained from Dr Delgado and purified as previously described [15] (link).
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