Log In Sign up
The largest database of trusted experimental protocols

Pyridoxine 5 phosphate

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Pyridoxine 5'-phosphate is a chemical compound that serves as a cofactor for various enzymatic reactions in the body. It is the phosphorylated form of pyridoxine, also known as vitamin B6. Pyridoxine 5'-phosphate plays a crucial role in the metabolism of amino acids, proteins, and lipids.

Automatically generated - may contain errors

Lab products found in correlation

Phenyl sepharose, by GE Healthcare (1 mentions) Protease inhibitor, by Merck Group (1 mentions) Thiamine, by Serva Electrophoresis (1 mentions) Pyridoxal, by ITW Reagents (1 mentions) Pyridoxal 5 phosphate, by Merck Group (1 mentions) Pentylenetetrazole, by Merck Group (1 mentions)

Spelling variants of this product

Pyridoxine (35 citations)

2 protocols using pyridoxine 5 phosphate

1

Bacterial Growth and Protein Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ingredients for bacterial growth and all reagents used for protein purification were from Sigma-Aldrich, except Phenyl-Sepharose, which was purchased from GE Healthcare. Pyridoxine 5'-phosphate was obtained from PLP (98% pure; Sigma-Aldrich) according to the method of Kazarinoff and McCormick (12 (link)).
Barile A., Battista T., Fiorillo A., di Salvo M.L., Malatesta F., Tramonti A., Ilari A, & Contestabile R. (2021). Identification and characterization of the pyridoxal 5’-phosphate allosteric site in Escherichia coli pyridoxine 5’-phosphate oxidase. The Journal of Biological Chemistry, 296, 100795.
+ Open protocol
+ Expand
2

Enzyme Activity Analysis Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents from the following manufacturers were used: thiamine—Serva Electrophoresis GmbH, Heidelberg, Germany; pyridoxal—PanReac AppliChem, Barselona, Spain; glycerol—Biomedicals, LLC, Santa Ana, CA, USA; pentylenetetrazole (PTZ), protease inhibitors, buffers and other reagents for the analysis of enzyme activities—Sigma-Aldrich, St. Louis, MO, USA. Pyridoxine-5′-phosphate was synthesized from pyridoxal-5′-phosphate (Sigma-Aldrich, St. Louis, MO, USA) as described previously [52 (link)]. The solutions were prepared in Milli-Q deionized water, the salts used were of the highest purity available.
Aleshin V.A., Graf A.V., Artiukhov A.V., Boyko A.I., Ksenofontov A.L., Maslova M.V., Nogués I., di Salvo M.L, & Bunik V.I. (2021). Physiological and Biochemical Markers of the Sex-Specific Sensitivity to Epileptogenic Factors, Delayed Consequences of Seizures and Their Response to Vitamins B1 and B6 in a Rat Model. Pharmaceuticals, 14(8), 737.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!