The largest database of trusted experimental protocols

Anti human ganglioside gd2 antibody

Manufactured by BioLegend
Sourced in United States

The Anti-human Ganglioside GD2 antibody is a laboratory reagent used for the detection and measurement of the GD2 ganglioside, a glycolipid molecule found on the surface of certain cells. This antibody can be used in various research applications, such as flow cytometry, immunohistochemistry, and ELISA.

Automatically generated - may contain errors

2 protocols using anti human ganglioside gd2 antibody

1

T-cell Phenotyping and Activation Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry surface staining of T cell phenotype and activation markers were performed after co-culture experiments using the following anti-human antibodies CD3 (OKT3, eBioscience, San Diego, CA, USA), CD3 (OKT3, SK7), CD4 (RPA-T4), CD8 (SK1), CD69 (FN50), CD137 (4B4-1), CD25 (BC96), and CD154 (24-31) (all BioLegend, San Diego, CA, USA). GD2 expression on target cells was analyzed via an anti-human Ganglioside GD2 antibody (BioLegend).
+ Open protocol
+ Expand
2

Simultaneous Apoptosis and GD2 Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enriched CD24-positive cells (~107) were simultaneously stained with Vybrant™ FLICA pan-Caspase Apoptosis Assay Kits for flow cytometry (Invitrogen/Thermo Scientific, Waltham, MA, USA) 1:150 and APC conjugated anti-human Ganglioside GD2 Antibody (BioLegend, San Diego, CA, USA) 1:100 in 0.25 mL of IsoFlow Sheath Fluid (Beckman Coulter, Pasadena, CA, USA) for 30 min at 37 °C. Then the cells were washed once with warm IsoFlow fluid and measured by a Sony SH800Z Sorting Flow Cytometer (Sony Biotechnology, Tokyo, Japan) with automatic channel compensation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!