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Anti human ganglioside gd2 antibody

Manufactured by BioLegend
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The Anti-human Ganglioside GD2 antibody is a laboratory reagent used for the detection and measurement of the GD2 ganglioside, a glycolipid molecule found on the surface of certain cells. This antibody can be used in various research applications, such as flow cytometry, immunohistochemistry, and ELISA.

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Lab products found in correlation

Sh 800z sorting flow cytometer, by Sony (1 mentions) Isoflow sheath fluid, by Beckman Coulter (1 mentions)

2 protocols using anti human ganglioside gd2 antibody

1

T-cell Phenotyping and Activation Profiling

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Flow cytometry surface staining of T cell phenotype and activation markers were performed after co-culture experiments using the following anti-human antibodies CD3 (OKT3, eBioscience, San Diego, CA, USA), CD3 (OKT3, SK7), CD4 (RPA-T4), CD8 (SK1), CD69 (FN50), CD137 (4B4-1), CD25 (BC96), and CD154 (24-31) (all BioLegend, San Diego, CA, USA). GD2 expression on target cells was analyzed via an anti-human Ganglioside GD2 antibody (BioLegend).
Zimmermann K., Kuehle J., Dragon A.C., Galla M., Kloth C., Rudek L.S., Sandalcioglu I.E., Neyazi B., Moritz T., Meyer J., Rossig C., Altvater B., Eiz-Vesper B., Morgan M.A., Abken H, & Schambach A. (2020). Design and Characterization of an “All-in-One” Lentiviral Vector System Combining Constitutive Anti-GD2 CAR Expression and Inducible Cytokines. Cancers, 12(2), 375.
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2

Simultaneous Apoptosis and GD2 Staining

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Enriched CD24-positive cells (~107) were simultaneously stained with Vybrant™ FLICA pan-Caspase Apoptosis Assay Kits for flow cytometry (Invitrogen/Thermo Scientific, Waltham, MA, USA) 1:150 and APC conjugated anti-human Ganglioside GD2 Antibody (BioLegend, San Diego, CA, USA) 1:100 in 0.25 mL of IsoFlow Sheath Fluid (Beckman Coulter, Pasadena, CA, USA) for 30 min at 37 °C. Then the cells were washed once with warm IsoFlow fluid and measured by a Sony SH800Z Sorting Flow Cytometer (Sony Biotechnology, Tokyo, Japan) with automatic channel compensation.
Mikhailov A, & Sankai Y. (2024). Apoptosis in Postmortal Tissues of Goat Spinal Cords and Survival of Resident Neural Progenitors. International Journal of Molecular Sciences, 25(9), 4683.
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