Log In Sign up
The largest database of trusted experimental protocols

Macrophage base medium xf

Manufactured by PromoCell
Visit Supplier

Macrophage Base Medium XF is a serum-free, chemically defined cell culture medium designed to support the growth and maintenance of human and murine macrophages in vitro. It provides the essential nutrients and growth factors required for the optimal proliferation and differentiation of macrophages.

Automatically generated - may contain errors

Lab products found in correlation

M1 macrophage generation medium xf, by PromoCell (2 mentions) Cd80 b7 1 monoclonal antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 dye, by Thermo Fisher Scientific (2 mentions) Macs multistand, by Miltenyi Biotec (1 mentions) Mini macs separation unit, by Miltenyi Biotec (1 mentions) Ms column, by Miltenyi Biotec (1 mentions)

3 protocols using macrophage base medium xf

1

Differentiation of PBMCs into M1 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen PBMCs from individual donors without cancer were obtained from UW-Carbone Cancer Center Biobank. PBMCs were differentiated into M0 and M1 macrophages using Macrophage Base Medium XF (Item No. C-28057) and M1-Macrophage Generation Medium XF (Item No. C-28055) from Promo Cell according to manufacturer’s instructions in NUNC 6-well cell culture plates (Item No. 140675). Macrophage CD80 was detected using CD80/B7–1 Monoclonal Antibody (Item No. 66406–1-IG) from Thermo Fisher conjugated to Alexa Fluor 488 Dye (immunofluorescence 1:100).
Hu Y., Manasrah B.K., McGregor S.M., Lera R.F., Norman R.X., Tucker J.B., Scribano C.M., Yan R.E., Humayun M., Wisinski K.B., Tevaarwerk A.J., O’Regan R.M., Wilke L.G., Weaver B.A., Beebe D.J., Jin N, & Burkard M.E. (2021). Paclitaxel induces micronucleation and activates pro-inflammatory cGAS-STING signaling in triple-negative breast cancer. Molecular cancer therapeutics.
+ Open protocol
+ Expand
2

Differentiation of Frozen PBMCs into M0 and M1 Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen PBMCs from individual donors without cancer were obtained from UW-Carbone Cancer Center Biobank. PBMCs were differentiated into M0 and M1 macrophages using Macrophage Base Medium XF (Item No. C-28057) and M1-Macrophage Generation Medium XF (Item No. C-28055) from Promo Cell according to manufacturer’s instructions. Macrophage CD80 was detected using CD80/B7–1 Monoclonal Antibody (Item No. 66406–1-IG) from Thermo Fisher conjugated to Alexa Fluor 488 Dye (immunofluorescence 1:100).
Hu Y., Manasrah B.K., McGregor S.M., Lera R.F., Norman R.X., Tucker J.B., Scribano C.M., Yan R.E., Humayun M., Wisinski K.B., Tevaarwerk A.J., O’Regan R.M., Wilke L.G., Weaver B.A., Beebe D.J., Jin N, & Burkard M.E. (2021). Paclitaxel induces micronucleation and activates pro-inflammatory cGAS-STING signaling in triple-negative breast cancer. Molecular cancer therapeutics.
+ Open protocol
+ Expand
3

Enrichment of CD11b+ Cells from GBM Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Starting from briefly cultured cells obtained from the T_Mass and the T_SVZ of 4 GBM patients (GBM 4, 7, 17, and 23) included in this study, immunomagnetic microbeads decorated with recombinantly engineered antibody fragments for CD11b (Miltenyi Biotec, Cat. No. 130-049-601) were used with the MiniMACS Separation Unit (Miltenyi Biotec, Cat. No. 130-042-102), MACS MultiStand (Miltenyi Biotec, Cat. No. 130-042-303) and MS Columns (Miltenyi Biotec, Cat. No. 130-042-201) to enrich for the CD11b+ cell fraction. Specifically, cell numbers were counted and incubated with CD11b microbeads for 15 min at 4°C. Following centrifugation and resuspension, cells were run through the columns using the separation unit. Upon immunomagnetic separation, CD11b-enriched cells were plated at approximately 104cells/cm2 in Macrophage Base Medium XF (PromoCell, Cat. No. C-28057) or M2-Macrophage Generation Medium XF (PromoCell, Cat. No. C-28056) prepared and supplemented with cytokines and mix as per the manufacturer’s instructions.
Licón-Muñoz Y., Avalos V., Subramanian S., Granger B., Martinez F., Varela S., Moore D., Perkins E., Kogan M., Berto S., Chohan M.O., Bowers C.A, & Piccirillo S.G. (2024). Single-nucleus and spatial landscape of the sub-ventricular zone in human glioblastoma. bioRxiv.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!