Fe2+ chloride (Sigma-Aldrich, 372,870) was dissolved in water to a final concentration of 10 μM. The tested compounds were added to 1 ml of Fe2+ chloride solution with a final concentration of 40 μM and incubated at room temperature for 10 min. Next, FerroZine Iron Reagent (Sigma-Aldrich, 160,601) was added with a final concentration of 20 μM and incubated at room temperature for 1 h [67 (link)]. The absorbance at 562 nm was measured using a microplate reader (Thermo Scientific, Varioskan Flash). Results were normalized to DMSO control group (DMSO vehicle; set as 100%).
Fe 2 chloride
Manufactured by Merck Group
Fe(II) chloride is an inorganic compound with the chemical formula FeCl2. It is a crystalline solid that is soluble in water. Fe(II) chloride is a common laboratory reagent used in various chemical applications.
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Lab products found in correlation
3 protocols using fe 2 chloride
1
Ferrous Iron Reduction Assay
2
Synthesis of iron(II) tris(2-pyridylmethyl)amine complex
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All reagents and solvents
were employed at the highest commercial quality and used without additional
purification. TPA and peracetic acid (36–40 wt % in acetic
acid, stored at 4 °C) were purchased from Sigma-Aldrich. Iron(II)
bis(trifluoromethanesulfonate)bis(acetonitrile), [Fe(OTf)2(CH3CN)2], was prepared according
to a literature procedure from anhydrous Fe(II) chloride (Sigma-Aldrich).54 (link) [FeII(TPA)(CH3CN)2)](OTf)2 was prepared by metalation of the
ligand TPA (Sigma-Aldrich) with [Fe(OTf)2(CH3CN)2] in dry CH3CN, and crystallization
was performed by slow diffusion of dry diethyl ether in a dry dichloromethane
solution as described in a literature method.55 (link) Preparation and handling of air-sensitive materials were performed
in an inert atmosphere by using a standard Schlenk and vacuum line
techniques or a glove bag under N2 atmosphere. Subsequently,
the complex was stored under inert atmosphere. When the [FeII(TPA)(OTf)2] complex is dissolved in CH3CN, two solvent molecules enter the iron first coordination sphere
giving rise to the [FeII(TPA)(CH3CN)2)](OTf)2 complex. From now on, solely the
cation [FeII(TPA)(CH3CN)2]2+ will be mentioned.
were employed at the highest commercial quality and used without additional
purification. TPA and peracetic acid (36–40 wt % in acetic
acid, stored at 4 °C) were purchased from Sigma-Aldrich. Iron(II)
bis(trifluoromethanesulfonate)bis(acetonitrile), [Fe(OTf)2(CH3CN)2], was prepared according
to a literature procedure from anhydrous Fe(II) chloride (Sigma-Aldrich).54 (link) [FeII(TPA)(CH3CN)2)](OTf)2 was prepared by metalation of the
ligand TPA (Sigma-Aldrich) with [Fe(OTf)2(CH3CN)2] in dry CH3CN, and crystallization
was performed by slow diffusion of dry diethyl ether in a dry dichloromethane
solution as described in a literature method.55 (link) Preparation and handling of air-sensitive materials were performed
in an inert atmosphere by using a standard Schlenk and vacuum line
techniques or a glove bag under N2 atmosphere. Subsequently,
the complex was stored under inert atmosphere. When the [FeII(TPA)(OTf)2] complex is dissolved in CH3CN, two solvent molecules enter the iron first coordination sphere
giving rise to the [FeII(TPA)(CH3CN)2)](OTf)2 complex. From now on, solely the
cation [FeII(TPA)(CH3CN)2]2+ will be mentioned.
3
Analytical Techniques for Organic Compound Characterization
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GC analyses were carried out on a Varian CP-3800 gas chromatograph equipped with a capillary methylsilicone column (30 m × 0.25 mm × 25 μm) Chrompack CP-Sil 5 CB. NMR spectra were recorded on a Bruker AC300 (300 MHz) spectrometer and were internally referenced to the residual proton solvent signal. HR-MS were acquired with a Thermo Scientific Orbitrap Fusion Mass Spectrometer equipped with an ESI ion source. UV analyses were carried out with HP HEWLETT PACKARD 8453 spectrophotometer. All reagents and solvents were purchased at the highest commercial quality and were used without further purification unless otherwise stated. Fe(CH3CN)2(OTf)2 (−OTf = CF3SO3−) was prepared according to a literature procedure12 (link) from Fe(ii ) chloride (Sigma Aldrich). Solvents were purchased from Sigma Aldrich and used as received.
5-Triisopropylsilyl-pyridine-2-carboxaldehyde11,13 (link) and 5-triisopropylsilyl-2-picolylamine11b were prepared according to literature procedure and spectral data were in accordance with those reported. Menthyl acetate14 (link) and 2,6-dimethylcyclohexanol15 (link) were prepared according to literature procedures and spectral data were in accordance with those reported.
5-Triisopropylsilyl-pyridine-2-carboxaldehyde11,13 (link) and 5-triisopropylsilyl-2-picolylamine11b were prepared according to literature procedure and spectral data were in accordance with those reported. Menthyl acetate14 (link) and 2,6-dimethylcyclohexanol15 (link) were prepared according to literature procedures and spectral data were in accordance with those reported.
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