Anti acetylated tubulin

Antibodies used were as follows: anti-YAP1, anti-phosphor-YAP1 (S127), and anti-acetyl-lysine were from Cell Signaling Technology (Danvers, MA, USA). Anti-PSME4, anti-histone H3, and anti-HDAC2 were from Abcam (Cambridge, UK). Anti-14-3-3 epsilon was from Thermo Fisher Scientific (Waltham, MA, USA). Anti-HSP90, anti-HDAC6, anti-acetylated-tubulin, and anti-GAPDH were from Santa Cruz Biotechnology (Dalla, CA, USA). Anti-actin, anti-tubulin, anti-HA, and anti-flag were from Sigma (St. Louis, MO, USA). Alexa Fluor 568-conjugated goat anti-mouse IgG was from Molecular Probes (Invitrogen, Waltham, MA, USA).
Reagents used were as follows: apicidin, cycloheximide, chloroquine, actinomycin D, leptomycin B, nicotinamide, tubastatin A, and doxycycline were from Sigma (St. Louis, MO, USA). MG132 was purchased from Cayman (Ann Arbor, MI, USA). Puromycin and blasticidin were from InvivoGen (San Diego, CA, USA).
siRNAs targeting HDAC6, FBXW7, SOCS6, and PSME4 were purchased from Dharmacon (Lafayette, CO, USA). Scramble siRNA was purchased from Bioneer (Daejeon, Korea).

Western-blots were performed as previously described [8 (link)]. The primary antibodies were: anti-Aurora A/AIK (rabbit monoclonal, 1:100, Cell Signaling Technology®), anti-Phospho-Aurora A (Thr288) (C39D8) (rabbit monoclonal, Cell Signaling Technology®), anti-GAPDH (6C5) (mouse monoclonal, Merck Millipore, Overijse, Belgium), anti-CXCR4 (C-20) (goat polyclonal 1:100, Santa Cruz®), anti-Vimentin (rabbit monoclonal 1:1000, Cell Signaling®), anti-F-actin (NH3) (mouse monoclonal, 1:500, Abcam®), anti-β-actin-peroxidase (mouse monoclonal, 1:10 000, Sigma®), anti-acetylated tubulin (mouse monoclonal 1:100, Santa Cruz®), anti-α-tubulin (mouse monoclonal 1:100, Santa Cruz®), anti-CDC42 (mouse monoclonal 1:100, Santa Cruz®, TX, USA). Western blot quantification was performed using the Image J software (n = 3).