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Goat anti rabbit igg antibody conjugated to alkaline phosphatase

Manufactured by Merck Group

Goat anti-rabbit IgG antibody conjugated to alkaline phosphatase is a lab equipment product. It is a secondary antibody that binds to rabbit primary antibodies, with an alkaline phosphatase enzyme attached. This allows for detection and quantification of target proteins in various immunoassay applications.

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2 protocols using goat anti rabbit igg antibody conjugated to alkaline phosphatase

1

Protein Detection Using Chemiluminescence

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N-ethylmaleimide (NEM) was purchased from Pierce (Rockford, IL). Duo-Lux chemiluminescent / fluorescent substrate was purchased from Vector Labs (Burlingame, CA). Anti-peroxiredoxin-2 antibody was purchased from Abcam (Cambridge, MA). Goat anti-rabbit IgG antibody conjugated to alkaline phosphatase was purchased from Millipore (Billerica, MA). Nitrocellulose (162-0112, 0.2μm pore size) was purchased from Bio-Rad (Hercules, CA). Percoll was purchased from GE healthcare life science (Pittsburgh, PA). All other materials were purchased from Sigma–Aldrich (St Louis, MO).
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2

Antibody Detection of Peroxiredoxin-2

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Antibodies against Peroxiredoxin-2 were purchased from Abcam (Cambridge, MA). Goat anti-rabbit IgG antibody conjugated to alkaline phosphatase was purchased from Millipore (Billerica, MA). All other materials were purchased from Sigma–Aldrich (St Louis, MO).
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